Mechanism of activated hepatic stellate cells promote angiogenesis of hepatocellular carcinoma
10.3877/cma.j.issn.2095-3232.2016.05.013
- VernacularTitle:活化态肝星状细胞促进肝癌血管新生的机制
- Author:
Yajun TANG
1
;
Chuzhi PAN
;
Yi LU
;
Zhaofeng TANG
;
Nan LIN
Author Information
1. 中山大学附属第三医院肝胆外科
- Keywords:
Carcinoma,hepatocellular;
Activited hepatic stellate cell;
Angiopoietin-1;
Angiogenesis
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2016;5(5):323-327
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the mechanism of activated hepatic stellate cells (aHSC) promote the angiogenesis of hepatocellular carcinoma (HCC).MethodsaHSCs were collected after being cultured for 1, 3, 5, 7 d respectively . The relative expression of aHSC angiopoietin-1 (Ang-1) messenger ribonucleic acid (mRNA) was detected by real-time fluorescence quantitative polymerase chain reaction (PCR). The expression of aHSC Ang-1 protein was observed by immunolfuorescence staining. The impact of aHSC on the proliferation of hepatic vascular endothelial cells (HVEC) was observed by Transwell chamber. The impact of aHSC on HVEC tube formation was observed by tube formation assay. The experimental data were compared using one-way analysis of variance and LSD-t test.ResultsAfter aHSC were cultured for 1, 3, 5, 7 d, the relative expression of aHSC Ang-1 mRNA was respectively 1.000±0.024, 1.920±0.080, 6.230±0.320 and 7.820±0.380, which gradually increased as the culture time went on (LSD-t=7.32, 13.68, 8.34;P<0.05). The immunolfuorescence staining showed that Ang-1 and smooth muscle actin antibody (aSMA) were co-expressed in the aHSC. Transwell chamber indirect co-culture showed that aHSC could promote the proliferation of HVEC, and the effect weakened after Ang-1 antibody was added. Tube formation assay showed that HVEC could polymerize and gradually developed tubular structure in the aHSC conditioned medium, and the effect signiifcantly weakened after Ang-1 antibody was added. ConclusionaHSC may promote the proliferation and angiogenesis of HVEC in HCC by secreting Ang-1.
