Construction of mouse model of liver implanted tumor after hepatectomy
10.3877/cma.j.issn.2095-3232.2016.03.015
- VernacularTitle:小鼠肝切除术后肝种植瘤模型的建立
- Author:
Junbin ZHANG
1
;
Genshu WANG
;
Jing ZHOU
;
Zhiying FENG
;
Huiling LIU
;
Xiaolong CHEN
;
Hui LI
;
Li WANG
;
Shihui LI
;
Jianxu YANG
Author Information
1. 中山大学附属第三医院器官移植中心
- Keywords:
Hepatectomy;
Carcinoma,hepatocellular;
Neoplasm recurrence,local;
Neoplasm seeding;
Mice
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2016;5(3):190-194
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the construction of mouse model of liver implanted tumor after hepatectomy by implantation of liver tumor tissue.MethodsH22 hepatocellular carcinoma (HCC) cells of mice were implanted to the scapula of 2 BALB/c (nu/nu) nude mice and tumors developed in 2 weeks. Eighteen nude mice were divided into three groups according to random number table method: non-hepatectomy group (group A), 30% hepatectomy group (group B) and 70% hepatectomy group (group C) with 6 mice in each group. The hepatectomy model of nude mice was established. The left lateral lobe and middle lobe were dissected for the nude mice in group A. The tissues of left lateral lobe were ligated and resected in group B. And the tissues of left lateralobe and middle lobe were ligated and resected in group C. The tumor tissues incised from the scapula were implanted to the livers of nude mice in three groups. The tumor formation rate and the volume of implanted tumors of three groups were compared. The tumor formation rate was compared using Fisher's exact probability test, and the volume of implanted tumors was compared using Kruskal-Wallis test and Bonferroni method.ResultsThe tumor formation rate of group A, B and C after 2 weeks was respectively 6/6, 6/6 and 5/6, and no signiifcant difference was observed (P=1.000). The median volume of implanted tumors in group A, B and C was respectively 19(6-33), 22(8-44) and 150(58-186) mm3. The median volume of implanted tumors in group C was signiifcantly larger than those in group A and B (Z=9.03, 7.12;P<0.0167). The implanted tumors in three groups were all conifrmed as HCC and with different degrees of necrosis and inlfammatory cell inifltration by histopathological examination.ConclusionsImplantation of liver tumor tissue can be used to construct an ideal mouse model of liver implanted tumor after major hepatectomy with the advantages of short experimental period, high tumor formation rate and good stability.
