Effect of Wnt signaling pathway activated on occurrence of hilar cholangiocarcinoma
10.3877/cma.j.issn.2095-3232.2015.05.016
- VernacularTitle:Wnt信号通路激活在肝门部胆管癌发生中的作用
- Author:
Jianpeng CAI
1
;
Li HUANG
;
Wei CHEN
;
Wenjie HU
;
Kunsong ZHANG
Author Information
1. 中山大学附属第一医院胆胰外科
- Keywords:
Bile duct neoplasms;
Signal transduction;
Microchip analytical procedures;
Wnt2 protein
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2015;(5):323-326
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of Wnt signaling pathway activated on occurrence of hilar cholangiocarcinoma.MethodsClinical data of 129 patients with hilar cholangiocarcinoma and 45 patients with congenital choledochal cyst undergoing surgical resection in the First Afifliated Hospital of Sun Yat-sen University between July 1998 and May 2007 were retrospectively studied. Among the 129 patients with hilar cholangiocarcinoma, 91 were males and 38 were females with the average age of (56±23) years old. Among the 45 patients with congenital choledochal cyst, 32 were males and 13 were females with the average age of (39±11) years old. The informed consents of all patients were obtained and the local ethical committee approval had been received. The tissue microarray of the pathological specimen was made. Wnt signal protein (Wnt) -2, β-catenin and T cell factor-4 (TCF-4) were treated by immunohistochemical staining. The positive expression of 3 indexes Wnt-2, β-catenin and TCF-4 observed simultaneously was considered as activated Wnt signaling pathway. The rate comparison of two groups was conducted using chi-square test.ResultsAccording to results of immunohistochemical staining,the positive expression of Wnt-2 in hilar cholangiocarcinoma tissues was observed in the appearance of brown granules in cytoplasm, while the positive expression of β-catenin and TCF-4 was observed in the appearance of brown granules in cell nucleus. The incidence of simultaneous positive expression of the 3 indexes Wnt-2, β-catenin and TCF-4 in hilar cholangiocarcinoma tissues was 54% (70/129), which was signiifcantly higher than 29% (13/45) in congenital choledochal cyst (χ2=11.2,P<0.05).ConclusionThe activation of Wnt signaling pathway may be one of the important molecular biological mechanisms of the occurrence of hilar cholangiocarcinoma.
