Expression and significance of HIF-1α and VEGF in liver tissues after ischemia-reperfusion injury in rats
10.3877/cma.j.issn.2095-3232.2015.05.014
- VernacularTitle:缺血-再灌注损伤大鼠肝组织HIF-1α和VEGF的表达及其意义
- Author:
Yuxiang HAN
1
;
Shanshan OU
;
Xiaoyu XIAO
;
Jingyu YAO
;
Lukun YANG
Author Information
1. 中山大学附属第五医院麻醉科
- Keywords:
Liver;
Reperfusion injury;
Hypoxia-inducible factor 1,alpha subunit;
Vascular endothelial growth factors;
Rats
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2015;(5):314-317
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the expression and significance of hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) in liver tissues after ischemia-reperfusion injury (IRI) in rats.MethodsSixteen Sprague-Dawley (SD) rats were randomized into the IRI group and the Sham group according to the random number table, 8 rats in each group. The portal vein and branches of hepatic artery of the rats in the IRI group were clamped with the atraumatic vascular clamp. After 45 min of ischemia, reperfusion was performed for 6 h. The porta hepatis of rats in the Sham group was exposed and the hepatic blood flow was not occluded. The morphological changes of the liver tissues in two groups were observed. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested and the contents of HIF-1α and VEGF in liver tissues were determined. Data comparison between two groups was conducted usingt test.ResultsUnder light microscope, swelling, vacuolar degeneration of the liver cells, inflammatory cell infiltration, hepatic sinusoidal dilatation, disorganized hepatic cords and spotty necrosis were observed in the IRI group. The histomorphology of liver tissues in the Sham group was normal. The serum ALT and AST in the IRI group was respectively (1 007±130) and (1 307±72) U/L, which were significantly higher than (59±4) and (81±3) U/L in the Sham group (t=20.700, 48.448;P<0.05). The relative expression of HIF-1α and VEGF in liver tissues of IRI group was 1.77±0.10 and 1.86±0.05 respectively, which were significantly higher than 0.60±0.22 and 0.83±0.06 in the Sham group (t=13.623, 35.563;P<0.05).ConclusionThe expression of HIF-1αand VEGF in liver tissues increase significantly during early IRI, which may play a role in reducing the IRI of organism.
