Inhibition of ENAH gene silenced by siRNA on growth of liver cancer cell
10.3877/cma.j.issn.2095-3232.2015.04.013
- VernacularTitle:siRNA沉默ENAH基因对肝癌细胞生长的抑制作用
- Author:
Qunai HUANG
1
;
Kunpeng HU
;
Bo LIU
;
Zhicheng YAO
;
Zhiyong XIONG
Author Information
1. 中山大学附属第三医院甲状腺乳腺外科
- Keywords:
Liver neoplasms;
Carcinogenicity tests;
Growth charts;
Nude mouse
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2015;(4):246-249
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the inhibition of ENAH gene silenced by siRNA on the growth of liver cancer cell.MethodsENAH-siRNA and control-siRNA were transfected to human liver cancer cell HCCLM3 using liposome Lipofectamine 2000 and ENAH-siRNA group and control-siRNA group were established. The expression of ENAH protein was detected by Western blot. Tumorigenic ability of the liver cancer cell was observed through nude mice tumorigenicity assay. Tumor volume was recorded, growth curve was drawn and survival analysis was conducted. The experimental data of two groups were compared usingt test, and the survival analysis was conducted using Kaplan-Meier method and Log-rank test.ResultsThe expression of ENAH protein in ENAH-siRNA group was significantly less than that in control-siRNA group. The formation time of solid tumor after inoculation in nude mice in ENAH-siRNA group was (24±3) d, which was significantly longer than (8±2) d in control-siRNA group (t=12.55,P<0.05). The median survival time of the nude micewas 64 (48~81) d in ENAH-siRNA group and was 34 (21~48) d in control-siRNA group. There was significant difference in the overall survival rate between two groups (χ2=14.33,P<0.05).Conclusion ENAH gene silenced by siRNA may obviously weaken the tumorigenic ability of liver cancer cell and inhibit the growth of tumor.
