Experimental study on siRNA silencing human cervical cancer oncogene 2 inhibits proliferation of cholangiocarcinoma cells
10.3877/cma.j.issn.2095-3232.2015.04.012
- VernacularTitle:siRNA沉默人宫颈癌基因2抑制胆管癌细胞增殖的实验研究
- Author:
Jing WANG
1
;
Jie BAO
;
Peng GUO
;
Di YAO
;
Hao HUANG
;
Ke HE
Author Information
1. 中山大学附属第一医院妇产科
- Keywords:
Bile duct neoplasms;
Human cervical cacontroler ocontrologene-2;
Cell proliferation;
Mouse
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2015;(4):242-245
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the influence of human cervical cancer gene 2 (HCCR-2) expression inhibited by siRNA on the proliferation of cholangiocarcinoma cells.MethodsPcmv6-AC-GFP/HCCR-2-siRNA lentiviral vector and Pcmv6-AC-GFP control vector were used to infect RBE human cholangiocarcinoma cells to establish the siRNA group and the control group. HCCR-2 protein expression was detected by Western blot.In vitro proliferation of cholangiocarcinoma cells was detected by MTT method andIn vivoproliferation of cholangiocarcinoma cells was detected by mice subcutaneous implanted tumor model. The experimental data of two groups were compared usingt test.ResultsThe average relative expression of HCCR-2 protein in RBE human cholangiocarcinoma cells of the siRNA group and the controlgroup was respectively 0.21±0.03 and 0.70±0.02. Compared with the control group, the relative expression of HCCR-2 protein of the siRNA group was signiifcantly reduced (t=-8.06,P<0.05). After 24 h and 48 h infection, the absorbance (A) measured in human cholangiocarcinoma cells of the siRNA group was respectively 0.05±0.01 and 0.16±0.01, which were signiifcantly lower than 0.11±0.02 and 0.39±0.06 of the control group (t=-8.80,-11.31;P<0.05). By the 30th day of subcutaneous implanted tumor grew, the tumor volume of the mice in the siRNA group was (106±28) mm3, which was signiifcantly smaller than (516±24) mm3 of the mice in the control group (t=-29.80,P<0.05).ConclusionExpression of HCCR-2 silenced by siRNA may inhibit the proliferation of cholangiocarcinoma cells.
