Effect of ABCG5 and ABCG8 gene expression on formation of cholesterol gallstone and cholesterol polyp
10.3877/cma.j.issn.2095-3232.2015.02.015
- VernacularTitle:ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉形成中的作用
- Author:
Liang DENG
1
;
Mingyi LIANG
;
Kunsong ZHANG
;
Fengfeng XU
;
Wenfeng LI
;
Jihong ZHANG
Author Information
1. 中山大学附属第一医院东院肝胆外科1
- Keywords:
Cholecystolithiasis;
Polyps;
Cholesterol;
ABCG5;
ABCG8;
Gene expression
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2015;(2):125-128
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate ABCG5 and ABCG8 gene in the occurrence and development of cholesterol gallstone and cholesterol polyp.MethodsClinical data of 60 patients who underwent cholecystectomy in the First Afifliated Hospital of Sun Yat-sen University from March 2012 to March 2014 were studied retrospectively. Of the 60 patients, 31 were males and 29 were females with a mean age of (49±6) years old. The informed consents of all patients were obtained and the local ethical committee approval had been received. According to the results of pathological diagnosis, the patients were divided into the cholesterol gallstone group (gallstone group), the cholesterol polyp group (polyp group) and the routine cholecystectomy group (control group). The relative expression of ABCG5 and ABCG8 mRNA in gallbladder epithelial cells was determined by real-time fluorescence quantitative polymerase chain reaction (PCR). The comparison of the relative mRNA expression between three groups were conducted using One-way analysis of variance and LSD-t test.ResultsThe relative expression of ABCG5 and ABCG8 mRNA was 3.3±0.9 and 6.9±1.5 respectively in gallstone group, which were signiifcantly higher than 2.4±0.6 and 4.3±1.5 in control group (LSD-t=23.58, 16.55;P<0.05). The relative expression of ABCG5 and ABCG8 mRNA was 2.6±0.7 and 4.6±1.3 respectively in polyp group and no signiifcant difference was observed when comparing with those in control group (LSD-t=1.18, 0.73;P>0.05).ConclusionABCG5 and ABCG8 gene may play a role in the occurrence and development of cholesterol gallstone and cholesterol polyp through different mechanisms.
