Impact of purinergic receptor P2Y12 gene inhibited by siRNA on the angiogenesis of hepatocellular carcinoma
10.3877/cma.j.issn.2095-3232.2015.01.015
- VernacularTitle:siRNA沉默P2Y12受体基因对肝细胞癌血管生成能力的影响
- Author:
Dezhi ZOU
1
;
Mingming GUO
;
Jianghui LIU
;
Ruibin CAI
;
Danping XU
Author Information
1. 中山大学附属第一医院急诊科
- Keywords:
Receptors,purinergic P2Y12;
Liver neoplasms,experimental;
Neovascularization,pathologic
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2015;(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of purinergic receptors P2Y12 gene inhibited by small interference RNA (siRNA) on the angiogenesis of hepatocellular carcinoma (HCC). Methods Human HCC cell line MHCC97H was infected using P2Y12-siRNA lentiviral expression vector and empty negative control vector to establish siRNA group and control group. The expression of P2Y12 protein in two groups was detected by Western blot assay. angiogenesis assay was used to deifne the angiogenesis potential of HCC cell. The microvessel density (MVD) of nude mice subcutaneous tumors was observed. The experimental data between two groups were compared using t test. Results The average relative expression of P2Y12 protein were 0.07±0.01 and 0.26±0.02 respectively in siRNA group and control group. The expression of P2Y12 protein in siRNA group decreased significantly compared with that in control group (t=-35.64, P<0.05). No continuous closed small tubular structure was observed in the human umbilical vein endothelial cell (HUVEC) stimulated by the supernatant of siRNA group, while large number of closed tubules was observed in the HUVEC stimulated by the supernatant of control group. A few microvessels of nude mice subcutaneous tumors were observed in siRNA group. The MVD was 5±1, which was signiifcantly lower than that in control group (23±6) (t=-17.01, P<0.05). Conclusion Purinergic receptors P2Y12 gene inhibited by siRNA can suppress the angiogenesis of HCC.
