Inhibition of HCCR-2 gene expression by siRNA suppresses liver metastasis of ovarian cancer
10.3877/cma.j.issn.2095-3232.2015.01.014
- VernacularTitle:siRNA沉默HCCR-2基因抑制卵巢癌肝转移的实验研究
- Author:
Jing WANG
1
;
Jianming LIANG
;
Anqi LIU
;
Wenjie CHEN
;
Mian HE
Author Information
1. 中山大学附属第一医院妇产科
- Keywords:
Neoplasms,unknown primary;
Liver neoplasms;
Ovarian neoplasms;
Human cervical cancer oncogene-2
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2015;(1):50-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of human cervical cancer oncogene-2 (HCCR-2) inhibited by small interference RNA (siRNA) on the liver metastatic potential of ovarian cancer. Methods High metastatic ability human ovarian cancer cell line HO-8910PM was infected using HCCR-2-siRNA lentiviral expression vector and empty negative control vector to establish small interference (SI) group and negative control (NC) group. The expression of HCCR-2 protein in two groups was detected by Western blot assay. Transwell assay was used to deifne the cell invasion ability. Liver metastases model of ovarian cancer was established to detect the cell metastatic ability. The data between two groups were compared using t test, and the rates were compared using Chi-square test. Results The average relative expression of HCCR-2 protein in SI group (0.16±0.02) signiifcantly decreased compared with that in NC group (0.86±0.05) (t=-8.06, P<0.05). The membrane permeating cell count in SI group (58±3) was signiifcantly less than that in NC group (262±19) (t=-37.77, P<0.05). The incidence of liver metastases in SI group (2/6) was signiifcantly lower than that in NC group (6/6) (P<0.05). The number of metastases in SI group (1.3 ± 0.2) was signiifcantly less than that in NC group (9.4 ± 0.8) (t=-10.25, P<0.05). Conclusion Inhibition of HCCR-2 gene expression by siRNA can reduce the invasion and liver metastatic potential of ovarian cancer cells.
