Protein kinase C-epsilon gene silenced by siRNA inhibits the development of cholangiocellular carcinoma and its mechanism
10.3877/cma.j.issn.2095-3232.2014.06.012
- VernacularTitle:siRNA沉默蛋白激酶Cε基因抑制胆管细胞癌生长及其作用机制
- Author:
Mingming GUO
1
;
Jianghui LIU
;
Ruibin CAI
;
Jing WANG
;
Bin CHEN
Author Information
1. 中山大学附属第一医院急诊科
- Keywords:
Bile duct neoplasms;
RNA interference;
Protein kinase c-epsilon;
Cell proliferation;
Cell apoptosis
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2014;(6):52-55
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of protein kinase C-epsilon (PKCε) gene silenced by small interfering ribonucleic acid (siRNA) in inhibiting the development of cholangiocellular carcinoma and its mechanism.MethodsHuman cholangiocellular carcinoma QBC939 cells were respectively transfected using PKCε-siRNA and negative control (NC)-siRNA to establish PKC group and NC group. And untransfected control (CTRL) group was established. Cell counting kit (CCK)-8 assay was used to define the cell proliferation inhibition rate. Cell apoptosis rate was detected by flow cytometery. The expressions of protein PKCε and survivin were detected by Western blot. The comparison of three groups was conducted using one way analysis of variance and pairwise comparison using LSD-t test.ResultsThe cell proliferation inhibition rates at 24, 48, 72 h [(7.52±0.33)%, (15.28±0.20)%, (37.12±0.45)% ] increased gradually in PKC group, where significant difference was observed compared with those in CTRL group (LSD-t=15.37, 27.12, 35.05;P<0.05). The cell apoptosis rate was (56.9±6.1)% in PKC group, which was significantly higher compared with that in CTRL group [(12.5±1.3) % ] (LSD-t=28.55,P<0.05). The expressions of protein PKCε and survivin decreased in PKC group compared with those in NC group and CTRL group.ConclusionPKCε gene silenced by siRNA may inhibit the development of cholangiocellular carcinoma through down-regulating the expression of protein survivin and promoting cell apoptosis.
