Tracking pulmonary metastasis of hepatocellular carcinoma of nude mouse imaging
10.3877/cma.j.issn.2095-3232.2014.05.012
- VernacularTitle:荧光成像技术活体示踪裸鼠肝细胞癌肺转移
- Author:
Zhiqiang YE
1
;
Xuhui LIU
;
Feilong WU
;
Kun ZHAO
;
Weidong PAN
Author Information
1. 中山大学附属第三医院急诊科
- Keywords:
Carcinoma,hepatocellular;
Liver neoplasms,experimental;
Mice,nude;
Luciferases,bacteria;
Live-imaging;
Tracking;
Neoplasm metastasis
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2014;(5):307-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of fluorescence imaging of labeling the hepatocellular carcinoma (HCC) cells with luciferase in tracking the pulmonary metastasis of HCC of nude mouse . Methods Low metastatic potential HCC97L and high metastatic potential HCC97H cells were transfected into HCC cell lines HCC97L-FL, HCC97H-FL with stable expression of luciferase. A total of 24 nude mice were randomly divided into HCC97L-FL group and HCC97H-FL group by the random number table method. Cell suspension was prepared by adding HCC97L-FL, HCC97H-FL cells (5×106) into the phosphate buffered saline (PBS, 50 μl) respectively. Cell suspension was injected into the caudal vein of nude mice. The model of tracking pulmonary metastasis of HCC with fluorescein was established. The gross and tissue samples of the pulmonary metastasis tracked by fluorescence imaging were observed.The data between two groups were compared using t test. Results Through fluorescence detection, intensive fluorescence could be observed in the HCC97L-FL, HCC97H-FL cells. After 2 to 3 weeks of modeling, fluorescence signal was collected from the nude mice lung in HCC97L-FL group. The fluorescence intensity enhanced gradually in 3-4 week. No fluorescence signal was observed in the nude mice lung in HCC97H-FL group. After 4 weeks of modeling, the fluorescence intensity was (1.73 ± 0.16) ×104 a.u. in HCC97H-FL group, which was significantly higher than that in HCC97L-FL group [(0.03 ±0.01) ×104 a.u.] (t=23.652, P<0.05). The nude mice lung in HCC97H-FL group was densely covered with metastatic tumors of varying sizes after 4 weeks of modeling. Some of the bigger tumors were visible on the lung surface. No metastatic tumor was observed in the HCC97L-FL group. The metastatic tumor was observed with an obvious cell nuclear atypia and characteristic feature of HCC. Conclusions Fluorescence imaging of using luciferase to label HCC cells can dynamically track the pulmonary metastasis of HCC of nude mouse . This technique provides a reliable quantitative research method for studying the growth, invasion and metastasis of HCC cells .
