Role of SIAH2 protein in the development of hepatocellular carcinoma
10.3877/cma.j.issn.2095-3232.2014.03.014
- VernacularTitle:SIAH2蛋白在肝细胞癌发生、发展中的作用研究
- Author:
Ruilei LIU
1
;
Jiani WANG
;
Peng ZHANG
;
Qiaochu ZHANG
;
Xi LI
;
Hua JIANG
;
Yong HUANG
Author Information
1. 中山大学附属第三医院甲乳外科
- Keywords:
Carcinoma,hepatocellular;
Cell proliferation;
Cell migration assays;
Neoplasm invasiveness;
Transfection;
Seven in absentia homolog
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2014;(3):189-193
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of SIAH2 protein in the occurrence and development of hepatocellular carcinoma (HCC). Methods Human HCC Bel-7404 cells in logarithmic growth phase were inoculated. Empty carrier small interference ribonucleic acid (siRNA) and SIAH2 siRNA were transfected in human HCC Bel-7404 cells. Then the cells were divided into 2 groups:control-siRNA group and SIAH2-siRNA group. Theβ-actin was taken as control, and the expression of SIAH2 protein in human HCC Bel-7404 cells of 2 groups was detected by Western Blot. The proliferation of cells in 2 groups was detected by methyl thiazolyl tetrazolium (MTT) assay. Cell migration in 2 groups was observed by cell scratch test. Cell invasion in 2 groups was observed by Transwell assay. The data in 2 groups were compared using t test. Results The average relative expression of SIAN2 in control-siRNA and SIAH2-siRNA group were 0.71±0.02, 0.33±0.01 respectively. The expression of SIAN2 in SIAH2-siRNA group decreased obviously compared with that in control-siRNA group (t=-4.629, P<0.05). The proliferation rate in SIAH2-siRNA group also decreased obviously. The cell migration rate in SIAH2-siRNA group[(14.3±0.4)%] was significantly lower than that in control-siRNA group [(45.3±0.4)%]( t=-3.689, P<0.05). The membrane permeating cell count in SIAH2-siRNA group (122±7) was signiifcantly less than that in control-siRNA group (563±10) (t=-3.428, P<0.05). Conclusion SIAH2 protein can promote the proliferation, migration and invasion of HCC cells and thus accelerate the occurrence and development of HCC.
