Research on the optimum reperfusion duration of liver ischemia-reperfusion injury model by occluding proper hepatic artery in rats
10.3877/cma.j.issn.2095-3232.2014.01.013
- VernacularTitle:大鼠肝固有动脉阻断肝缺血-再灌注损伤模型最佳灌注时间的研究
- Author:
Ying LIN
1
;
Huiling LIU
;
Bing WANG
;
Lixian ZENG
;
Huixin HE
;
Zhuofu WEN
;
Genshu WANG
;
Bin WU
Author Information
1. 中山大学附属第三医院消化内科
- Keywords:
Hepatic artery;
Reperfusion injury;
Models,animal;
Apoptosis;
Cell proliferation
- From:
Chinese Journal of Hepatic Surgery（Electronic Edition）
2014;(1):52-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the optimum reperfusion duration of liver ischemia-reperfusion injury (IRI) model by occluding the proper hepatic artery in rats. Methods Fourty-eight Sprague Dawley (SD) rats with mean weight of (200±25) g were randomly assigned to 8 groups by random number table method:IRI-0, 1, 3, 6, 12, 24, 48 h and sham operation (SO) group with 6 rats in each group. The proper hepatic arteries in rats of IRI groups were selectively occluded for 1 h and then blood lfow recovered. Samples of blood and liver tissues were collected at the time points of 0, 1, 3, 6, 12, 24, 48 h of reperfusion. In SO group, samples were collected after the proper hepatic artery was isolated and the ifrst portal was exposed for 1 h. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), pathological changes, DNA fragmentation rates, and levels of Ki-67 expression of liver tissues were observed in each group. Measurement data of multiple groups were compared using one-way analysis of variance and LSD-t test. Results The levels of serum ALT in IRI-0, 1, 3, 6, 12, 24, 48 h and SO group were (53±25), (85±20), (96±18), (411±96), (87±19), (81±15), (46±6), (60±14) U/L respectively. The levels of serum AST were (238±63), (364±111), (375±68), (1 291±511), (800±87), (854±218), (484±219), (248±94) U/L accordingly. The levels of serum ALT, AST in IRI 6 h group were the highest (F=36.015, 18.241;P<0.05). The damage of liver tissues in IRI 6 h group was the most serious. The DNA fragmentation rates of liver tissues were (7.5±1.5)%, (9.2±2.2)%, (9.3±2.3)%, (12.6±2.4)%, (6.3±1.0)%, (5.4±0.9)%, (4.5±0.8)%, (4.5±1.1)%accordingly, which was the highest in IRI 6 h group (F=15.992, P<0.05). The levels of Ki-67 expression of liver tissues were (3.5±1.4), (5.6±1.8), (8.7±2.3), (13.7±2.4), (15.2±1.2), (20.5±2.2), (31.8±2.5), (2.4±1.2)/high power ifeld accordingly, which was the highest in IRI 48 h group (F=261.707, P<0.05). Conclusions The liver IRI model can be successfully established by occluding the proper hepatic artery, and the optimum reperfusion duration of IRI is 6 h.
