Epithelial-mesenchymal phenotypes analysis of circulating tumor cells with dual-antibody for the prognosis assessment in hepatocellular carcinoma
10.3760/cma.j.cn113884-20240701-00196
- VernacularTitle:肝细胞癌患者循环肿瘤细胞双抗体检测与分型对预后的影响
- Author:
Huizhong ZHANG
1
;
Fazhuang FANG
;
Xiaodong ZHANG
;
Junjie LIU
;
Jianming FANG
;
Jingchao TANG
;
Haiyang LI
;
Yibiao ZHANG
Author Information
1. 浙江金华广福肿瘤医院肝胆胰外科，金华 321000
- Keywords:
Carcinoma, hepatocellular;
Circulating tumor cell;
Epithelial-mesenchymal typing;
Recurrence;
Prognosis
- From:
Chinese Journal of Hepatobiliary Surgery
2024;30(11):830-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the feasibility of circulating tumor cell (CTC) dual-antibody enrichment and dual-antibody detection for epithelial-mesenchymal phenotypes in patients with hepatocellular carcinoma (HCC), and investigate the clinical diagnostic value of CTC typing in evaluating postoperative recurrence and prognosis of HCC.Methods:Of 89 HCC patients who underwent surgical treatment in Zhejiang Jinhua Guangfu Tumor Hospital from March 2020 to January 2024 were enrolled into this study, including 73 males and 16 females, aged (64.4±9.5) years old. The peripheral blood samples of patients were collected before operation. Epithelial CTC, mesenchymal CTC and hybrid epithelial-mesenchymal CTC in blood samples of patients with HCC were enriched and detected by EpCAM/CSV double capture antibodies and PanCK/CSV double detection antibodies. Kaplan-Meier analysis was employed to assess recurrence-free survival (RFS) and overall survival (OS) rates. Univariate and multivariate Cox regression were used to analyze the effects of different types of CTC on postoperative RFS and OS.Results:The detection rates of total CTC, epithelial CTC, mesenchymal CTC and hybrid epithelial-mesenchymal CTC were 92.1% (82/89), 64.0% (57/89), 62.9% (56/89) and 55.1% (49/89), respectively. Multivariate Cox regression analysis showed that HCC patients with more mesenchymal CTC ( HR=2.408, 95% CI: 1.580-3.668) and hybrid epithelial-mesenchymal CTC ( HR=1.840, 95% CI: 1.004-3.371) in peripheral blood had higher postoperative recurrence risk (both P<0.05). Univariate Cox regression showed patients with more total CTC ( HR=1.426, 95% CI: 1.040-1.954, P=0.028) was associated with survival. Conclusion:The technique of epithelial-mesenchymal phenotypes analysis of circulating tumor cells based on dual-antibody capture and dual-antibody detection is feasible. The number of mesenchymal CTC and hybrid epithelial-mesenchymal CTC before operation is the influencing factor of postoperative recurrence in patients with hepatocellular carcinoma.
