Analysis of oral microflora diversity and distribution in patients of long-term survival after liver transplantation
10.3760/cma.j.cn113884-20240613-00180
- VernacularTitle:长期存活肝移植患者口腔微生物群多样性及分布特点分析
- Author:
Chengrun ZHANG
1
;
Siyuan WEI
;
Shaocheng LYU
;
Zuyu WANG
;
Chaoran YU
;
Dongxue ZHANG
;
Haiyan WANG
Author Information
1. 首都医科大学附属北京朝阳医院肝胆外科，北京 100020
- Keywords:
Liver transplantation;
Oral microbiome;
16S rDNA;
High-throughput sequencing
- From:
Chinese Journal of Hepatobiliary Surgery
2024;30(11):824-829
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the composition of oral microbiome in patients of long-term survival after liver transplantation, and compare the diversity and distribution of oral microbiome in liver transplant patients with or without liver cancer.Methods:This is a cross-sectional study. Clinical data of 20 patients of long-term survival after liver transplantation from Beijing Chaoyang Hospital Affiliated to Capital Medical University from July 2023 to October 2023 were continuously collected, including 13 males and 7 females, aged 55 (48, 60) years. There were eight patients with hepatocellular carcinoma (HCC) undergoing liver transplantation and 12 non-HCC liver transplant patients. Oral microbiome was analyzed by 16S rDNA gene sequencing, and the diversity analysis and LEfSe analysis were performed.Results:The top ten groups of bacteria in the HCC and non-HCC liver transplant patients were both Bacteroidota, Firmicutes, Proteobacteria, Actinobacteria, Fusobacteriota, Patescibacteria, Campylobacterota, Cyanobacteria, spirochaetota, and Aenigmarchaeota. The top ten genus levels of bacteria in the HCC and non-HCC liver transplant patients were both Haemophilus, Porphyromonas, Gemella, Fusobacterium, Actinomyces, Prevotella, Rothia, Streptococcus, Neisseria. By LEfSe analysis, we found the enrichment of Flavobacteriales, Flavobacteriaceae, Capnocytophaga, Mogibacterium_timidum, Capnocytophaga_leadbetteri, Campylobacter_showae in the HCC group, while the enrichment of Veillonella_atypica, Prevotella_histicola in the non-HCC group.Conclusion:The main microbiome was similar between HCC and non-HCC liver transplant patients, but there were also some differences in microbiome communities.
