Clinical significance of expression of MUC5B and PDCD4 protein in intrahepatic cholangiocellular carcinoma
10.3760/cma.j.cn113884-20240601-00169
- VernacularTitle:MUC5B与PDCD4蛋白在肝内胆管细胞癌中表达的临床意义
- Author:
Jinhai LI
1
;
Fujing CAI
;
Huawei ZHAI
;
Yu YANG
;
Guangzheng SUN
;
Haifeng ZHANG
;
Minghui ZHU
;
Yue LIN
;
Shenghua PAN
;
Shuqun LI
Author Information
1. 温州医科大学附属第三医院肝胆外科，温州 325200
- Keywords:
Nomogram;
Intrahepatic cholangiocellular carcinoma;
Mucin 5B;
Programmed cell death 4;
Immunohistochemistry
- From:
Chinese Journal of Hepatobiliary Surgery
2024;30(10):755-760
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression characteristics of mucin 5B (MUC5B) protein and programmed cell death factor 4 (PDCD4) protein in patients with intrahepatic cholangiocarcinoma (ICC), and to construct a nomogram model for prognosis prediction.Methods:Clinical data of 100 patients who underwent radical surgical resection and were diagnosed as ICC by postoperative pathology from September 2009 to September 2020 in the Third Affiliated Hospital of Wenzhou Medical University were retrospectively selected, including 46 males and 54 females, aged (56.9±12.2) years old. Immunohistochemistry was used to detect the expression of MUC5B and PDCD4 protein in 100 cases of ICC and corresponding adjacent tissues respectively, and the relationship between them and clinicopathological factors of ICC patients was analyzed. Univariate and multivariate Cox regression analysis were performed to analyze the influencing factors on postoperative prognosis of ICC patients. The nomogram model was constructed using rms package and performed internal verification.Results:The positive expression rate of MUC5B protein in ICC was 76.0% (76/100), which was higher than that in para-cancer tissues 27.0%(27/100), and the difference was statistically significant ( χ2=11.33, P=0.015). While the positive expression rate of PDCD4 protein in ICC was 21.0%(21/100), which was lower than that in normal tissues 73.0% (73/100), and the difference was statistically significant ( χ2=15.57, P=0.007). Multivariate Cox regression analysis showed that ICC patients with carbohydrate antigen 19-9>37 kU/L, tumor length>5 cm, tumor TNM stage Ⅱ/Ⅲ, tumor medium/low differentiation, MUC5B positive expression, and PDCD4 negative expression had a high risk of short survival after resection (all P<0.05). The nomogram model was constructed based on the above indicators, and the C-index was 0.801. The postoperative survival calibration curve showed that the high predictive survival fit of the nomogram model, and the area under the receiver operating characteristic curve was 0.862. Conclusions:Positive expression of MUC5B protein and negative expression of PDCD4 protein in ICC tissue suggest poor prognosis of ICC patients. The nomogram model constructed on the basis of MUC5B and PDCD4 protein is well distinguished and has ideal predictive efficacy.
