Effects of 2 650 MHz radiofrequency radiation on the behavior and hippocampal neurotransmitter release of mice
10.3760/cma.j.cn112271-20231225-00221
- VernacularTitle:2 650 MHz射频辐射对小鼠行为学和海马神经递质释放的影响
- Author:
Yujie LIU
1
;
Jun WANG
;
Keqin LI
;
Chenxu CHANG
;
Ying LIU
;
Hongyan ZUO
;
Yang LI
;
Hong YANG
;
Yanhui HAO
;
Hua DENG
Author Information
1. 佛山科学技术学院生命科学与工程学院，佛山 528225
- Keywords:
Radiofrequency radiation;
Cognition;
Social behavior;
Emotion;
Neurotransmitter
- From:
Chinese Journal of Radiological Medicine and Protection
2024;44(5):354-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of 2 650 MHz radiofrequency (RF) exposure on the behavior and neurotransmitter release of mice.Methods:Adult male C57BL/6N mice were divided into a normal control (CON) group and a radiofrequency radiation (RFR) group using the random number table method. The mice in the RFR group were subjected to single-dose whole-body exposure to a uniform 2 650 MHz RF electromagnetic field for 3 h. During the RF exposure, the field strength in the effective working area of the RF radiation platform was measured using an electromagnetic radiation analyzer, and the changes in the anal temperature of the mice were monitored using an optical fiber thermometer. Moreover, the changes in the cognition, social interaction, and emotion of the mice were determined through the new object recognition test, social preference test, and open field test. Finally, the changes in the hippocampal neurotransmitter release levels of the mice were detected using microdialysis sampling and mass spectrometry, and the changes in the hippocampal tissue structure and ultrastructure were observed via microscopy.Results:Under the test conditions, RF radiation improved the anal temperature of the mice, with a maximum increasing amplitude of 0.61℃, falling within the range of thermal safety. The mice in the RFR group experienced a significant decrease in the frequency and time for exploring new objects ( t=4.50, 2.53, P < 0.05) in the new object recognition test, a significant decrease in the frequency ( t=0.08, P<0.01) and time ( t=0.03, P<0.05) for exploring other mice in the social preference test, and no significant change in the frequency and time for exploring the central area ( P > 0.05) in the open field test. Compared to the CON group, the RFR group showed an increase in the release of 5-hydroxytryptamine (5-HT) ( t=-2.56, P < 0.05) and a decrease in the release of acetylcholine (ACh) ( t=2.21, P < 0.05), no significant difference in the release of glutamate (Glu) and γ-aminobutyric acid (GABA) ( P > 0.05), and no evident damage to the hippocampal tissue and structure and synaptic ultrastructure. Conclusions:2 650 MHz RF radiation may induce cognitive impairment and abnormal social preference in mice, which is attributed to neuronal dysfunctions and neurotransmitter release disorders under RF exposure.
