Protective Effect of Tanshinone on Injuried Cultured PC12 Cellsin Vitro
10.3321/j.issn:1001-5302.2001.06.020
- VernacularTitle:丹参酮对培养PC12细胞损伤模型的保护作用
- Author:
Li-Na HE
1
;
Jun YANG
;
Yi JIANG
;
Jing WANG
;
Chao LIU
;
Su-Bing HE
Author Information
1. Biological Medicinal Central Laboratory of Anhui
- From:
China Journal of Chinese Materia Medica
2001;26(6):413-416
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effects of tanshinone on ischemia-like injury models. Method:Six ischemia models including hypoxia, hypoglucose, oxidant injury, calcium overload, nitric oxide neurotoxicity and glutamic acid injury were used to assay the anti-ischemic roles of tanshinone in cultured PC12 cells, by using morphological examination, MTT staining and LDH measurement. Result: It was found that 3.125～200 μl*ml-1 tanshinone possessed obvious protective effects on PC12 cells from six ischemia-like injury models . Tanshinone could increase the number of life cells and decrease LDH activity significantly, particularly in hypoxia and caffeine injured model.Conclusion:Tanshinone protected PC12 cells from all injury models effectively in vitro. Its action may mainly focus on inhibiting development of the primary period of ischemia injury and calium overloading injury.
