Studies on Tissue Culture of Dendrobium chrysotoxum Lindl in Vitro
10.3321/j.issn:1001-5302.2001.06.005
- VernacularTitle:鼓槌石斛组织培养研究
- Author:
Hong XU
1
;
Jun LIU
;
Zheng-Tao WANG
;
De-Ran XU
;
Jia-Yi DING
Author Information
1. China Pharmaceutical University
- From:
China Journal of Chinese Materia Medica
2001;26(6):378-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective：To set up a system for the culture of Dendrobium chrysotoxum in vitro. Method：Tissue culture，fire fly luminescence and phenol-H2SO4 method. Result：The embryo could germinate with or without light, the MS, 1/2MS, B5, N6 mediums are suitable to the growth and the differentiation of sprout with light, 0.5 mg*L－1NAA and 1 mg*L－16-BA, and ATP have regular changes, the content of polysaccharide was 2.833% in plant and 7.254% in sprout. Conclusion：The light has no effects on the embryo germination, but the phytohormone，nitrogen source and organized elements are important to the growth and differentiation of the sprout which should be transferred to the MS, 1/2MS, B5, N6 mediums in time supplemented with NAA与6-BA, ATP may be served as the dynamic indication of nourishment demand in the plant. The content of polysaccharide in the sprout is higher and can be utilized.
