Studies on Difference between Sporoderm-broken and Nonbroken Spores of Ganoderma lucidum (Leyss. ex Fr.) Karst.by Polysaccharide Analysis
10.3321/j.issn:1001-5302.2001.05.014
- VernacularTitle:赤芝孢子粉破壁前后多糖释放能力比较研究
- Author:
Xing-Feng BAO
1
;
Ji-Nian FANG
Author Information
1. Shanghai Institute of Materia Medica, Academia Sinica
- From:
China Journal of Chinese Materia Medica
2001;26(5):326-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the release ability of water-soluble polysaccharides in sporoderm-broken and nonbroken spores of Ganoderma lucidum, and establish a comparatively correct method for the determination and analysis of polysaccharide contents in Chinese herbs.Method:The release ability of water-soluble polysaccharides was determined on the basis of phenol-sulfuric acid modification in different conditions.Result:The release ability of polysaccharides of sporoderm-broken spores was much grerter than that of nonbroken spores; and the phenol-sulfuric acid modified cation method proved excellent in accuracy and reproducibility, with a relative error less than 1.5%.Conclusion:The spores should be wall-wracked if used as a nutriment, or for extraction and analysis of their effective components. The method can be successfully used for the determination of polysaccharide contents in Chinese herbs or nutriments.
