1001-5302(2001)02-0128-04in Splenocytes in AA Rats
- VernacularTitle:六味地黄汤对佐剂性关节炎大鼠脾脏细胞表达细胞因子的影响
- Author:
Jian FANG
1
;
Yong-Xiang ZHANG
;
Xiang-Bin RU
;
Xiao-Long WEI
Author Information
1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences
- From:
China Journal of Chinese Materia Medica
2001;26(2):128-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The effect of Liuwei Dihuang decoctio n (LW) on the function of splenic T helper cells (Th) was studied and compared w ith those of the immune inhibitors such as cyclophosphamide (Cy) and cyclosporin A (CsA) in AA rats. Methods: The expression of mRNA for IFN- γ,IL-2, IL-4 and IL-10 in splenic T-Lymphocytes of adjuvant arthritis (AA) rats was evaluated using reverse transcription polymerase chain reaction (RT-PC R) technique. Results: mRNA expression level of IL-2 had a te ndency to elevate, but the mRNA expression levels of IFN-γ,IL-4 and IL-10 we re significantly decreased in AA rats in comparison with control groups. LW trea tment significantly inhibited the mRNA expression of IL-2 and promoted the expr ession of IFN-γ,IL-4 and IL-10 in splenocytes of AA rats, with an obvious ch aracteristic as compared with that of Cy or CsA. Conclusion: L W can correct the imdifferent balance of the functions of splenocyte Th1/Th2 in AA rats.
