Effects of Baicalin on Hepatocyte ApoptosisInduced by TNF-α and Act D in Rats
- VernacularTitle:黄芩苷对大鼠肝细胞凋亡的影响
- Author:
Cong HU
1
;
Ju-Qiang HAN
;
Zheng XU
;
Jing-Dong LIU
;
Yong HAO
Author Information
1. Bethune Medical College of PLA
- From:
China Journal of Chinese Materia Medica
2001;26(2):124-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To stuby the effects of baicalin on r at hepatocyte apoptosis induced by tumornecrosis factora (TNF-α) and actinomyc in D (Act D) in vitro. Methods: Hepatocytes were cultared with different concentrations of baicalin and the cell apoptosis was induced by TNF -α and Act D in vitro. After culturing for 24 h, the activity of hepatocyts wa s examined by MTT assay and the function of hepatocytes for secreting albumin w as detected by bromocresolum Viride method. Agarose gelelectrophoresis and flowc ytometric analysis were used to determine the apoptotic cells. Results: The activety of the hepatocyte (A) and content of albumin (ALB) in the supematant of cultured cells in the groups of 0.2 μg*ml-1 and 2.0 μg* ml-1 of baicaline were significantly higher than those in apoptotic model group (A:P<0.01; ALB:P<0.01);respectively Even the ALB value in the grou p of 0.2 μg*ml-1 baicalin was higher that of black control group (P< 0.01); The clear “ladder-like” streak-band of DNA fragments was foundonly in the apoptosis model group by the agarose gel electrophoresis. The flow cytomet ry showed that all the apoptosis rates in the three baicalin groups were lower t han that of the apoptotic model group (P<0.01)。Conclusion: Baicaline can restrain rat hepatocyte apoptosis induced by TNF-α and ActD in vitro.
