Effects of Musk Glucoprotein on the Function of Rat Polymorphonuclear Leukocytes Activated by IL-8 in Vitro
10.3321/j.issn:1001-5302.2001.01.018
- VernacularTitle:麝香糖蛋白成分对白细胞介素-8激活的大鼠中性白细胞功能的影响
- Author:
Wen-Jie WANG
1
;
Jin-Ye BAI
;
Gui-Fang CHENG
;
XIU-YUAN ZHU
Author Information
1. Institute of Materia Medica, CAMS and PUMC
- From:
China Journal of Chinese Materia Medica
2001;26(1):50-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on some functions of rat polymorphonuclear leukocytes activated by IL-8 in vitro.Method：An in vitro incubation system was used. Superoxide anion production was determined by cytochrome C reduction. β-glucuronidase and lysozyme release was quantitated by enzyme reactions in which phenolphthaleinglucuronic acid and Micrococcus Lysodeikticus were as the substrates, respectively.Results:In comparison with control, musk-1 at concentration 1～100 μg*ml-1 can increase superoxide anion production by 91.7%～291%, and decrease β-glucuronidase and lysozyme release by 2.2%～58.1% and 3.9%～39.8%, respectively.Conclusion:Inhibition of lysosomel enzyme release might be considered as one of mechanisms of antiinflammatory action of musk.
