Interaction between Th1 Cytokine and Th2 Cytokine on the Expression of Chemokine and Adhesion Molecule in Human Mesangial Cells .
- Author:
Kyoung Sun NA
1
;
Tae Young KANG
;
Jin Sook LEE
;
Yong Wook PARK
;
Hye Soon LEE
;
Wan Sik UHM
;
Tae Hwan KIM
;
Jae Bum JUN
;
Sang Cheol BAE
;
Tchun Yong LEE
;
Dae Hyun YOO
Author Information
1. Division of Rheumatology, Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea. dhyoo@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Cytokines;
Chemokines;
Adhesion molecules;
Mesnagial cell
- MeSH:
Autoimmune Diseases;
Chemokine CCL5;
Chemokines;
Cytokines;
Flow Cytometry;
Glomerulonephritis;
Humans*;
Immunoenzyme Techniques;
Inflammation;
Intercellular Adhesion Molecule-1;
Interleukin-10;
Interleukin-4;
Lupus Nephritis;
Mesangial Cells*;
Nephrectomy;
RNA, Messenger;
Up-Regulation
- From:The Journal of the Korean Rheumatism Association
2004;11(2):77-89
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Predominance of IFN-gamma over Th2 cytokines is evident in proliferative lupus nephritis. Th2 cytokines such as IL-10, or IL-4 have been known to suppress Th1 cytokine driven pro-inflammatory responses. However a combination of cytokines can exert variable effects during the evolution of autoimmune diseases depending on the various factors, such as the stage of diseases or local versus systemic expression. To determine whether Th2 cytokines play a pro- or anti-inflammatory role in the local glomerular inflammation induced by IFN-gamma, we studied the effect of IL-10 and IL-4 on the expression of chemokines and adhesion molecules in human mesangial cells stimulated with IFN-gamma. MEHTODS: Human mesangial cells were obtained from 3 patients undergoing nephrectomy. MCP-1, RANTES, ICAM-1, and CD40 expressions were examined in response to various cytokine stimulation; IFN-gamma, IL-10, IL-4, IFN-gamma+IL-10, IFN-gamma+IL-4, and IFN-gamma+IL-10+IL-4, respectively. Expression of MCP-1 mRNA was analyzed by RT-PCR and the levels of MCP-1 and RANTES in the culture supernatants were measured using an enzyme immunoassay. ICAM-1 and CD40 surface expressions were analyzed by flow cytometry. RESULTS: IFN-gamma increased basal mRNA expression of MCP-1, and IL-10 strongly enhanced the level of MCP-1 mRNA and protein expressions induced by IFN-gamma. IL-4 did not affect significantly on the MCP-1 expression induced by IFN-gamma. Although IFN-gamma increased the concentration of RANTES, IL-10 and IL-4 did not affect IFN-gamma induced RNTES expression. Increased expression of ICAM-1 and CD40 induced by IFN-gamma were not down-regulated by IL-10 or IL-4. CONCLUSION: Taken together, IL-10 appears to augment local inflammatory reaction though the up-regulation of MCP-1 from mesangial cells in the presence of IFN-gamma rather than inhibit inflammatory response in the pathogenesis of proliferative glomerulonephritis.
