Discussion on the Effects of Angelicae Sinensis Radix and Astragali Radix Extract on Coronary Microvascular Disease Based on NLRP3-mediated Pyroptosis
10.19879/j.cnki.1005-5304.202401055
- VernacularTitle:基于NLRP3介导的细胞焦亡探讨当归黄芪超滤物对冠状动脉微血管疾病的影响
- Author:
Chunyan YAN
1
;
Hugang JIANG
;
Xinqiang WANG
;
Kai LIU
;
Yingdong LI
;
Xinke ZHAO
Author Information
1. 甘肃中医药大学中西医结合学院,甘肃 兰州 730000
- Keywords:
Angelica Sinensis Radix and Astragali Radix extract;
coronary microvascular disease;
NLRP3 inflammasome;
pyroptosis;
HUVEC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(12):120-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Angelica Sinensis Radix and Astragali Radix extract(ASR-AR)on HUVEC pyroptosis;To explore its mechanism of treating coronary microvascular disease.Methods HUVEC were divided into blank group,model group,MCC950 group,ASR-AR low-,medium-and high-dosage groups.After modeling and treatment with drug containing serum,cell viability was detected by CCK-8 method,and cell apoptosis was detected by flow cytometry,phalloidin staining was used to detect cytoskeletal morphology,immunofluorescence staining was used to detect the expressions of VEGF,eNOS,Ang-2,ROS,ET-1 and TXA2,ELISA was used to detect the contents of IL-1β and IL-18 in cell supernatant,Western blot was used to detect the expressions of NLRP3,ASC,Caspase-1 and GSDMD protein in cells.Results Compared with the blank group,the model group showed a decrease in HUVEC cell viability(P<0.01)and an increase in cell apoptosis rate(P<0.01),cellular microfilament structure was in disorder and knotting,the expressions of VEGF and eNOS decreased,and expressions of Ang-2,ROS,ET-1 and TXA2 increased,the contents of IL-1β and IL-18 in cell supernatant increased(P<0.01),and the expressions of NLRP3,ASC,Caspase-1 and GSDMD protein in cells increased(P<0.01).Compared with the model group,ASR-AR low-,medium-and high-dosage containing serum could increase cell viability(P<0.05),decrease cell apoptosis rate(P<0.05),improve cell microfilament structure,elevate VEGF and eNOS expressions,decrease Ang-2,ROS,ET-1,TXA2 expressions,reduce IL-1β and IL-18 contents in cell supernatant(P<0.05),and decrease NLRP3,ASC,Caspase-1 and GSDMD protein expressions(P<0.05).ASR-AR medium-dosage group was more obvious(P<0.05).Conclusion ASR-AR can inhibit pyroptosis of HUVEC induced by AngⅡ,attenuate endothelial cell dysfunction,thus treating coronary microvascular disease,and its mechanism may be related to the inhibition of the assembly of NLRP3 inflammasome.
