Exploration on the Molecular Mechanism of Shenling Baizhu Powder in Improving Adipose Tissue Inflammation in Children with Obesity Based on Network Pharmacology and Animal Experimental Verification
10.19879/j.cnki.1005-5304.202406328
- VernacularTitle:基于网络药理学探讨参苓白术散改善儿童肥胖脂肪组织炎症的分子机制及动物实验验证
- Author:
Changmiao NIU
1
;
Liting LIANG
;
Yongkai YIN
;
Xiaoning YAN
;
Kaixi ZHANG
;
Gengtian NIU
Author Information
1. 山西中医药大学第一临床学院,山西 晋中 030619
- Keywords:
Shenling Baizhu Powder;
network pharmacology;
children obesity;
inflammation;
metabolic disorders;
experimental verification
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(12):27-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effective components and possible targets and mechanism of Shenling Baizhu Powder in improving adipose tissue inflammation in children with obesity with the help of network pharmacology and molecular docking method;To conduct preliminary verification through animal experiments.Methods The active components and targets of Shenling Baizhu Powder were screened through the TCMSP database.GeneCards,OMIM,DisGeNET databases were used to obtain the disease targets.An active component-target network was constructed by taking the intersection of drug and disease targets.Protein-protein interaction networks were constructed and core targets were obtained for GO and KEGG pathway enrichment analysis.Molecular docking validation was performed to key components and core targets.Animal experiments were conducted by establishing a model of young obese rats induced by high fat diet,and Shenling Baizhu Powder were given for gavage.The contents of TG and TC in serum and mRNA expressions of TNF-α,IL-1β,INF-γ and Fas in rat adipose tissue of epididymis were detected.Results The key components of Shenling Baizhu Powder to improve the microinflammation of obesity in children were piperlonguminine,acacetin,luteolin,denudatin B,mandenol,inermine,etc.There were 515 common drug-disease targets,and the core targets were TP53,SRC,PIK3R1,HSP90AA1 and PIK3CA.The results of GO enrichment analysis included inflammatory response,positive regulation of MAPK cascade,membrane raft,protein serine/threonine/tyrosine kinase activity,etc.KEGG pathway enrichment analysis results included metabolic,immune,endocrine,cancer and related liver disease signaling pathways.The results of molecular docking showed that the key components could play a regulatory role on the core target.Animal experiments showed that Shenling Baizhu Powder could improve microinflammation and metabolic indexes of model rats.Conclusion Shenling Baizhu Powder can act on multiple targets through various active components and multiple signaling pathways,adjusting inflammatory response and immune process,alleviating insulin resistance,regulating glucose and lipid metabolism,thereby improving adipose tissue inflammatory in children with obesity.
