Effects of Buyang Huanwu Decoction Mediating Cav1 in Regulating Wnt Pathway on Neuronal Cell Apoptosis in Cerebral Ischemia Mice
10.19879/j.cnki.1005-5304.202405323
- VernacularTitle:补阳还五汤介导Cav1调控Wnt通路对脑缺血小鼠神经细胞凋亡的影响
- Author:
Yin OUYANG
1
,
2
;
Fanzuo ZENG
;
Zhenkui LIU
;
Bowei CHEN
;
Yingfei LIU
;
Jian YI
;
Fengming TIAN
;
Yaqian XU
;
Baiyan LIU
Author Information
1. 湖南中医药大学第一附属医院,湖南 长沙 410000
2. 湖南省中医药研究院,湖南 长沙 410000
- Keywords:
Buyang Huanwu Decoction;
cerebral ischemia;
apoptosis;
Cav1;
Wnt signaling pathway;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(11):104-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Buyang Huanwu Decoction on neuronal cell apoptosis after cerebral ischemia based on mediating Cav1 in regulating Wnt pathway.Methods Male wild-type(WT)and Cav1-/-(KO)C57BL/6 mice were randomly divided into sham-operation group,model group and Buyang Huanwu Decoction group(18.5 g/kg).Cerebral ischemia model was prepared using middle cerebral artery occlusion method,and drug intervention was given for 14 days.Neurobehavioral score was performed,HE staining was used to observe the morphology of ischemic cortical area of brain tissue,TUNEL staining was used to detect neuronal apoptosis in ischemic cortical area,immunohistochemistry was used to detect the expressions of apoptosis related proteins and Wnt1,glycogen synthase kinase 3β(GSK3β)and β-catenin protein in ischemic cortical area.Results Compared with the same genotype sham-operation group,the neurobehavioral score of the model group mice significantly increased,neuronal cells in the ischemic cortical area showed vacuolar changes,with nuclear condensation and widened intercellular spaces,the apoptosis rate of nerve cells significantly increased,with increased expressions of Bax,GSK3β and decreased expressions of Bcl-2,Wnt1 and β-catenin(P<0.01).Compared with the same genotype model group,the neurobehavioral score of mice in Buyang Huanwu Decoction group were significantly decreased,the pathological damage of the ischemic cortical area improved,the apoptosis rate of nerve cells decreased,the expressions of Bax and GSK3β decreased,and the expressions of Bcl-2,Wnt1 and β-catenin increased(P<0.01).Compared with the WT model group,the KO model group showed an increase in neurobehavioral score,aggravated damage in ischemic cortical area,significantly increased neuronal apoptosis rate,and increased expression of GSK3β(P<0.05).Compared with the WT Buyang Huanwu Decoction group,the KO Buyang Huanwu Decoction group showed an increase in neurobehavioral score,aggravated damage in ischemic cortical area,significantly increased neuronal apoptosis rate,increased expressions of Bax and GSK3β,and decreased expressions of Bcl-2,Wnt1 and β-catenin(P<0.01).Conclusion Buyang Huanwu Decoction can inhibit neuronal cell apoptosis after cerebral ischemia,and its mechanism may be related to regulating the expressions of apoptosis-related proteins by mediating Cav1 to regulate the Wnt signaling pathway.
