Excavation of the Active Components and Potential Mechanisms of Mori Cortex-Lycii Cortex Intervention in Acute Lung Injury with Network Pharmacology Combined with Experimental Validation
10.19879/j.cnki.1005-5304.202404039
- VernacularTitle:网络药理学结合实验验证挖掘桑白皮-地骨皮干预急性肺损伤的活性成分及潜在机制
- Author:
Tianyu ZHANG
1
;
Zhenqi WU
;
Guanghua LIU
;
Da ZHAO
;
Xiyu ZHAO
;
Xuejie YU
;
Xiangyu LIANG
;
Zhaodong QI
Author Information
1. 辽宁中医药大学第一临床学院,辽宁 沈阳 110847
- Keywords:
Mori Cortex-Lycii Cortex;
acute lung injury;
network pharmacology;
molecular docking;
mechanism;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(11):42-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To validate the mechanism of Mori Cortex-Lycii Cortex(MCLC)in intervening acute lung injury(ALI)based on network pharmacology,molecular docking combined with animal experiments.Methods The TCMSP database was used to obtain the active components of MCLC;the SwissTargetPrediction database was used to predict the targets of active components;the GeneCards database and DisGeNET database were used to collect the disease targets of ALI;the key targets were screened by constructing a PPI network,and the key targets were subjected to GO and KEGG pathway enrichment;a drug-component-target-pathway network was constructed using Cytoscape software;AutoDock and PyMOL software were used to validate the molecular docking of some of the compounds and targets;LPS was used to establish a mouse model of ALI for experimental validation,and experimental validation was performed to main targets and pathways.Results Totally 44 active components of MCLC and 138 action targets were obtained;26 potential targets of MCLC intervention in ALI were obtained,mainly TNF,EGFR,NFKB1,MPO,TNFRSF1A,NOX4,etc.,and the key pathways were MAPK signaling pathway,IL-17 signaling pathway,NF-κB signaling pathway,etc.;molecular docking results showed that the core active components of MCLC and the main targets had strong binding activities;animal experiments showed that MCLC at medium and high dosages could effectively improve the lung histopathological damage in ALI mice,decrease the contents of IL-6 and TNF-α in serum(P<0.01),and increase IL-10 content(P<0.01);MCLC inhibited protein expressions of EGFR,PI3K,AKT,NF-κB p65 in lung tissue(P<0.01).Conclusion MCLC may intervene ALI by components such as quercetin and buddleoside,acting on targets including EGFR and TNF,through ulti-pathways of EGFR/PI3K/NF-κB signaling pathway,etc.
