Study on the Mechanism of Mild Moxibustion in Treating Primary Dysmenorrhea Rats Based on the Regulation of TRPV1 Expression Based on cAMP-PKA Signaling Pathway
10.19879/j.cnki.1005-5304.202403253
- VernacularTitle:基于cAMP-PKA信号通路调控TRPV1表达研究温和灸治疗原发性痛经大鼠作用机制
- Author:
Yanqiu SUN
1
,
2
;
Yulei LIANG
;
Di WANG
;
Liyun YANG
;
Xuanping ZHANG
;
Yan ZHANG
;
Xuan ZHANG
;
Yanxue XING
;
Min ZHOU
;
Xiaoyan WANG
;
Xinhua LI
Author Information
1. 河北中医药大学针灸推拿学院,河北 石家庄 050200
2. 河北中医针灸优势病症国际联合研究中心,河北 石家庄 050200
- Keywords:
primary dysmenorrhea;
mild moxibustion;
cAMP-PKA signaling pathway;
TRPV1;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(10):96-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of the treatment of primary dysmenorrhea(PD)by mild moxibustion on"Shenque"and"Guanyuan"acupoints based on the regulation of cAMP-PKA signaling pathway on TRPV1.Methods Totally 32 female non-pregnant Wistar rats were randomly divided into blank group,model group,mild moxibustion group and capsazepine group,with 8 rats in each group.Except for the blank group,the other groups all used estradiol benzoate intraperitoneal injection combined with ice water bath to establish a PD cold-dampness stagnation syndrome rat model.Intervention began on the first day of modeling,the mild moxibustion group selects"Shenque"and"Guanyuan"for mild moxibustion,20 min per time,the capsazepine group was injected capsazepine 2 mg/kg,once a day for 10 consecutive days.ELISA was used to detect uterine PGF2α and cAMP content,immunofluorescence staining was used to detect TRPV1 expression in uterine tissue,Western blot was used to detect PKA,p-PKA and TRPV1 protein expression.Results Compared with the blank group,the latency period of body twisting in the model group rats decreased,and the body twisting score increased(P<0.01);the contents of PGF2α and cAMP in uterine tissue increased(P<0.01),and the expressions of TRPV1 and p-PKA proteins increased(P<0.01).Compared with the model group,the mild moxibustion group and capsazepine group showed an increase in the latency period of body twisting and a decrease in the body twisting score(P<0.01);the content of PGF2α and cAMP in uterine tissue decreased(P<0.01),and the expressions of TRPV1 and p-PKA proteins decreased(P<0.05,P<0.01).Compared with the mild moxibustion group,the capsazepine group showed an increase in the latency period of body twisting and a decrease in the body twisting score(P<0.01);the contents of PGF2α and cAMP in uterine tissue decreased(P<0.05,P<0.01),and the expressions of TRPV1 protein decreased(P<0.05).Conclusion Mild moxibustion at"Shenque"and"Guanyuan"acupoints has obvious analgesic effect on PD rats,and its mechanism may be related to the regulation of uterine cAMP-PKA signaling pathway mediated TRPV1 protein expression.
