Exploration on the Mechanism of Jiegengbai Powder in the Treatment of Lung Adenocarcinoma Based on Network Pharmacology and Animal Experiments
10.19879/j.cnki.1005-5304.202401424
- VernacularTitle:基于网络药理学和动物实验探讨桔梗白散治疗肺腺癌作用机制
- Author:
Chao YUAN
1
;
Jiachen JIANG
;
Siqi KONG
;
Jianqing LIANG
;
Yi ZHANG
;
Yue ZHANG
;
Yu LIU
;
Jintian LI
Author Information
1. 甘肃中医药大学,甘肃 兰州 730000
- Keywords:
Jiegengbai Powder;
cisplatin;
chemotherapy;
lung adenocarcinoma;
network pharmacology;
PTEN;
PI3K/Akt/mTOR signaling pathway
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(9):33-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the targets and mechanism of Jiegengbai Powder in the treatment of lung adenocarcinoma based on network pharmacology and animal experiments.Methods The targets of effective components of Jiegengbai Powder were obtained from TCMSP,the targets of lung adenocarcinoma were screened from GeneCards,PharmGKB,DrugBank,TTD,OMIM databases,and the intersection targets were obtained.The protein-protein interaction(PPI)network and active components of Chinese materia medica-target network were constructed by using Cytoscape 3.8.0 software,and the key components and core targets were screened out.The intersection targets were analyzed by GO and KEGG enrichment analysis.PyMOL and AutoDockTools 1.5.6 software were used to verify the molecular docking between the key components and core targets.The lung cancer mice model was established.The mice were randomly divided into blank group,model group,cisplatin group,Jiegengbai Powder combined with cisplatin group,Jiegengbai Powder low-,medium-and high-dosage groups.After 14 days of intervention,the tumor inhibition rate was calculated,and the morphology of tumor tissues was observed by HE staining.The gene and protein expressions of PI3K,PTEN,Akt and mTOR in tumor tissues were detected by RT-qPCR and Western blot.Results The core targets of Jiegengbai Powder in the treatment of lung adenocarcinoma such as TP53,CASP3,BCL2L1 and AKT1 were screened by network pharmacology.The key pathways of enrichment were PI3K-Akt signaling pathway and so on.Jiegengbai Powder can inhibit the growth of tumor effectively.Compared with the model group,the mRNA expressions of PI3K,Akt and mTOR decreased in the Jiegengbai Powder medium-and high-dosage groups,and PTEN mRNA expression increased,the ratio of p-PI3K/PI3K,p-Akt/Akt and p-mTOR/mTOR decreased,and the expression of PTEN protein increased(P<0.05,P<0.01).Conclusion Jiegengbai Powder has the characteristics of multi-level and multi-target in the treatment of lung adenocarcinoma.It may promote tumor cell apoptosis and autophagy by regulating PI3K/Akt/mTOR signaling pathway,so as to achieve the anti-tumor effect of inhibiting tumor cell growth.
