Evaluation of the Quality of Cinnamomi Cortex from Different Origins Based on QAMS Multi-Component Quantitative Analysis Combined with Multivariate Statistical Analysis Technology
10.19879/j.cnki.1005-5304.202402163
- VernacularTitle:基于一测多评法多成分定量结合多元统计分析技术评价不同产地肉桂品质
- Author:
Min WANG
1
;
Tanjun WEI
;
Fei CHEN
;
Chunlong WANG
;
Cheng XIAO
;
Xu WEI
;
Lijie LU
;
Yi WANG
Author Information
1. 达州市中西医结合医院,四川 达州 635000
- Keywords:
Cinnamomi Cortex;
quality evaluation;
HPLC;
PCA;
OPLS-DA;
EW-TOPSIS
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(8):133-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for simultaneous determination of 14 components in Cinnamomi Cortex;To comprehensively evaluate its quality by multivariate statistical analysis technology.Methods HPLC method was used to establish the relative correction factors(RCF)between protocatechuic acid and 13 other components of coumalic acid,coumarin,cinnamyl alcohol,cinnamic acid,2-methoxycinnamic acid,cinnamaldehyde,o-methoxycinnamaldehyde,vanillic,syringic acid,4-hydroxybenzoic acid,procyanidin B2,epicatechin and procyanidin C1 to be measured,and the content of each component was calculated.The QAMS method was validated using external standard method(ESM),and the comprehensive quality of Cinnamomi Cortex was evaluated using PCA,OPLS-DA and EW-TOPSIS methods.Results The methodological verification results of ESM were in accordance with the requirements;the RCF established with protocatechuic acid as the reference had good durability under different experimental conditions,and the difference in content of each component between ESM and QAMS was not significant(P>0.05);the results of multivariate statistical analysis showed that accumulative contribution rate of former 2 principle components was 90.312%.Cinnamaldehyde,2-methoxycinnamic acid,procyanidin B2 and 4-hydroxybenzoic acid had a significant impact on the quality of Cinnamomi Cortex.The results of EW-TOPSIS showed that the Pi value of 16 batches of Cinnamomi Cortex ranged from 0.147 2 to 0.768 1.The quality of Cinnamomi Cortex from different producing areas varied greatly,and the overall quality of Cinnamomi Cortex from Guangxi Province was the best,followed by that from Guangdong Province and Yunnan Province,and the quality of Cinnamomi Cortex from Fujian Province was relatively poor.Conclusion The established QAMS multi-component quantitative method combined with multivariate statistical analysis technology can be used for comprehensive quality evaluation of Cinnamomi Cortex.
