Study on Quality Evaluation of Notopterygii Rhizoma et Radix through UPLC Fingerprint Combined with Chemometrics Analysis and Multi Index Component Content Determination
10.19879/j.cnki.1005-5304.202310552
- VernacularTitle:UPLC指纹图谱结合化学计量学分析和多指标成分含量测定的羌活药材质量评价研究
- Author:
Guangming HE
1
;
Xiaoying LU
;
Tianrui XIA
;
Feifei XIE
;
Rui LUO
;
Weisheng LYU
;
Yueyi LIANG
;
Zhenyu LI
;
Xiuzhi LI
;
Jianxiang ZHANG
;
Xiangdong CHEN
;
Dongmei SUN
Author Information
1. 广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,广东 佛山 528244
- Keywords:
Notopterygii Rhizoma et Radix;
fingerprint;
multi index component;
content determination;
UPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(8):126-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the ultra-high performance liquid chromatography(UPLC)chromatographic fingerprint of Notopterygii Rhizoma et Radix;To determine the contents of ferulic acid,nodakenin,ammijin,notopterol,isoimperatorin and volatile oil of Notopterygii Rhizoma et Radix from different producing areas;To provide reference for quality evaluation of Notopterygii Rhizoma et Radix.Methods Waters BEH C18 chromatographic column(2.1 mm×150 mm,1.7 μm)was used,with mobile phase acetonitrile-0.02%formic acid aqueous solution gradient elution,flow rate 0.25 mL/min,column temperature 25℃,detection wavelength 330 nm,injection volume 2 μL.UPLC fingerprints of 25 batches of Notopterygii Rhizoma et Radix were established,and the similarity analysis and chemometrics analysis were carried out.The contents of ferulic acid,nodakenin,ammijin,notopterol and isoimperatorin were determined simultaneously,and the contents of volatile oil was determined by steam distillation method.Results Totally 23 common fingerprint peaks were calibrated,11 known components were identified.According to the results of the cluster analysis and principal component analysis,25 batches of Notopterygii Rhizoma et Radix samples were divided into 3 categories,and the 6 potential differential components were screened out by orthogonal partial least squares-discriminant analysis(OPLS-DA).The results showed that the contents of notopterol and volatile oil from Sichuan Province were higher than those from Gansu Province and Qinghai Province.Conclusion The method established in the study is accurate and reliable,which can provide scientific basis and reference for the quality evaluation and control of Notopterygii Rhizoma et Radix.
