Effects of Quyu Jiedu Prescription on Iron Autophagy in Eutopic Endometrium of Endometriosis Rats
10.19879/j.cnki.1005-5304.202402093
- VernacularTitle:祛瘀解毒方对子宫内膜异位症大鼠在位子宫内膜铁自噬的影响
- Author:
Yiming LIU
1
;
Zhihong LIU
;
Xiaoliang LIU
;
Fang LIAN
Author Information
1. 山东中医药大学第一临床医学院,山东 济南 250000
- Keywords:
endometriosis;
Quyu Jiedu Prescription;
iron autophagy;
NCOA4;
eutopic intima;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(8):103-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Quyu Jiedu Prescription on iron autophagy in eutopic endometrial tissue of rats with endometriosis(EMS);To explore its mechanism in treating EMS.Methods A total of 50 female SD rats were randomly divided into blank group,model group,Western medicine group,Quyu Jiedu Prescription high-and low-dosage groups,with 10 rats in each group.Except for the blank group,all other groups were modeled using autologous transplantation method,and corresponding solutions were given by gavage for 4 weeks.HE staining was used to observe the morphology of eutopic endometrial tissue,the reagent kit was used to detect the content of ferrous ions(Fe2+)in eutopic endometrial tissue,Western blot was used to detect the protein expressions of nuclear receptor coactivator factor 4(NCOA4),ferritin heavy chain 1(FTH1),LC3BⅡ/Ⅰ and p62 in eutopic endometrial tissue,the mRNA expressions of NCOA 4,FTH1,LC3B and p62 were detected by qPCR,immunofluorescence colocalization was used to detect the co-localization of NCOA4 and LC3B in eutopic endometrial tissue.Results Compared with the blank group,the content of Fe2+in eutopic endometrial tissue of the model group decreased(P<0.01),the expression of NCOA4 protein and LC3B Ⅱ/Ⅰ ratio decreased(P<0.01),the expressions of FTH1 and p62 protein increased(P<0.05,P<0.01),the expression of NCOA4 mRNA decreased(P<0.01),the expression of p62 mRNA increased(P<0.01),and the co-localization of NCOA4-LC3B decreased(P<0.01).Compared with the model group,the content of Fe2+in eutopic endometrial tissue of each administration group increased(P<0.05,P<0.01),the expressions of FTH1 and p62 protein in Western medicine group decreased(P<0.05,P<0.01),the ratio of LC3B Ⅱ/Ⅰ in Quyu Jiedu Prescription high-dosage group increased(P<0.01),the expression of FTH1 and p62 protein decreased(P<0.05,P<0.01),the expression of NCOA4 protein and LC3B Ⅱ/Ⅰ ratio increased in Quyu Jiedu Prescription low-dosage group(P<0.05),while the expression of p62 protein decreased(P<0.05),the expression of NCOA4 mRNA increased in each administration group(P<0.01),and the co-localization of NCOA4-LC3B increased(P<0.05,P<0.01).There was no significant change in the morphology of eutopic endometrial tissue in each group.Conclusion Quyu Jiedu Prescription may activate the iron autophagy pathway mediated by NCOA4 to restore the activity of ferritin protein,increase the iron content,correct the imbalance of iron homeostasis in eutopic intima of EMS rats,and thus inhibit the development of EMS.
