Correlation Study on Changes in Chemical Composition and Anti-inflammatory Effects of Fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle
10.19879/j.cnki.1005-5304.202312517
- VernacularTitle:炒白芍-炙甘草配伍前后化学成分与抗炎作用相关性研究
- Author:
Yixin YE
1
;
Jialu SHANG
;
Guoyun CAI
;
Hua YIN
Author Information
1. 浙江中医药大学药学院,浙江 杭州 311402
- Keywords:
fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle;
compatibility;
chemical composition;
anti-inflammatory effect;
correlation;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(7):122-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigated the correlation between the effects of the compatibility of fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle on chemical composition and anti-inflammatory effects.Methods An HPLC-DAD method was employed for the simultaneous determination of various components of fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,including paeoniflorin,albiflorin,benzoylpaeoniflorin,oxypaeoniflorin,glycyrrhizic acid and glycyrrhizin.An adjuvant-induced arthritis rat model was established.Administration groups were given fried Paeoniae Radix Alba,Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,and fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle liquid by gavage for 2 weeks,respectively.Enzyme-linked immunosorbent assay was conducted to detect the levels of inflammatory factors IL-1β,TNF-α and PGE2 in serum of each group.Factorial design,bivariate correlation analysis,and multiple linear regression were employed to explore the effect of compatibility on chemical composition and its correlation with anti-inflammatory efficacy.Results The established HPLC-DAD analysis method revealed a significant increase in the content of oxypaeoniflorin,albiflorin and benzoylpaeoniflorin following the compatibility of fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle(P<0.05).Compared with the model group,the contents of IL-1β,TNF-α and PGE2 in serum of each administration group decreased(P<0.01),and the contents of IL-1β,TNF-α and PGE2 in serum of compatibility group were lower than fried Paeoniae Radix Alba group and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle group(P<0.05,P<0.01).The results from the factorial design indicated the presence of an interaction effect between fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle.Bivariate correlation analysis results showed negative correlations between oxypaeoniflorin,albiflorin,paeoniflorin,glycyrrhizin,benzoylpaeoniflorin and glycyrrhizic acid with the contents of IL-1β,TNF-α and PGE2,respectively.Multiple linear regression results revealed that glycyrrhizin and glycyrrhizic acid were negatively correlated with the contents of IL-1β,TNF-α and PGE2.Conclusion The established method for analyzing multiple constituents in the compatibility of fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle is straightforward,rapid,highly specific,accurate,and reliable,suitable for quality evaluation and control.The compatibility of fried Paeoniae Radix Alba-Glycyrrhizae Radix et Rhizoma Praeparata cum Melle can inhibit the expressions of IIL-1β,TNF-α and PGE2,and the content of chemical composition is correlated with anti-inflammatory effects.
