Effects of Simo Decoction on the Gastrointestinal Dynamics of Rats with Functional Dyspepsia of Liver and Spleen Stagnation Syndrome
10.19879/j.cnki.1005-5304.202306344
- VernacularTitle:四磨汤对肝脾气滞型功能性消化不良大鼠胃肠动力的影响
- Author:
Sainan ZHOU
1
;
Qian LUO
;
Qin LIU
;
Haiyue ZHANG
;
Xiaoyuan LIN
Author Information
1. 湖南中医药大学第一附属医院,湖南 长沙 410007
- Keywords:
Simo Decoction;
functional dyspepsia;
liver and spleen stagnation;
gastrointestinal dynamics;
vasoactive intestinal polypeptide;
vasoactive intestinal peptide receptor 2;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(6):117-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Simo Decoction on the expressions of vasoactive intestinal peptide(VIP)and its receptor 2(VIPR2)in rats with functional dyspepsia(FD)of liver and spleen stagnation syndrome;To investigate its mechanism of action in regulating gastrointestinal motility.Methods Totally 60 SD rats were randomly divided into blank group,model group,mosapride group(0.305 mg/kg)and Simo Decoction high-,medium-and low-dosage groups(5.62,2.81,1.40 g/kg).The liver and spleen qi stagnation FD rat model was prepared by multifactorial modeling method,and the corresponding drugs were given by gavage for 14 days.The general conditions of rats in each group were observed,and body mass,gastric emptying rate and small intestine propulsion rate were measured;the histological structure of gastric antrum tissue and duodenal tissue were observed by HE staining;the positive expressions of VIP and VIPR2 in duodenal tissue were observed by immunohistochemistry;the protein and mRNA expressions of VIP and VIPR2 in duodenal tissue were detected by Western blot and RT-PCR,respectively.Results Compared with the blank group,the general condition of the rats in model group was poorer,body mass,gastric emptying rate and small intestine propulsion rate were significantly lower(P<0.01),and the protein and mRNA expressions of VIP and VIPR2 in duodenal tissue significantly increased(P<0.05,P<0.01).Compared with the model group,the body mass,gastric emptying rate and small intestine propulsion rate in Simo Decoction high-and medium-dosage groups and the mosapride group significantly increased(P<0.05,P<0.01),and the protein and mRNA expressions of VIP and VIPR2 in duodenal tissue significantly decreased(P<0.05,P<0.01).HE staining showed no significant changes in the morphology of gastric antrum and duodenum tissues.Conclusion Simo Decoction may promote gastrointestinal motility by inhibiting the expressions of VIP and VIPR2 in duodenal tissue of FD rats with liver and spleen qi stagnation,thereby improving FD.
