Effect of Marsdeniae Tenacissimae Caulis on Human Osteosarcoma Cells Based on JAK1/STAT3 Signaling Pathway
10.19879/j.cnki.1005-5304.202306200
- VernacularTitle:基于JAK1/STAT3信号通路的通关藤对人骨肉瘤细胞增殖的影响
- Author:
Xiaochuan XUE
1
,
2
;
Junjun CHEN
;
Lingyan XU
;
Lanyi WEI
;
Yujie HU
;
Yangyun ZHOU
;
Mengyue WANG
;
Yonglong HAN
Author Information
1. 上海海洋大学食品学院,上海 200135
2. 上海交通大学医学院附属第六人民医院,上海 200030
- Keywords:
Marsdeniae Tenacissimae Caulis;
osteosarcoma;
proliferation;
apoptosis;
migration;
JAK1;
STAT3
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(6):108-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and potential mechanisms of Marsdeniae Tenacissimae Caulis(Tongguanteng)injection and extract in human osteosarcoma cells proliferation,migration,invasion,and apoptosis.Methods MNNG/HOS,Saos-2 osteosarcoma cells,and normal bone marrow mesenchymal stem cells(BMSC)were cultured in vitro.Cells were incubated with different concentrations of Tongguanteng injection and Tongguanteng extract(40,60,80 mg/mL).Cell proliferation was evaluated by CCK-8 assay and plate colony formation assay.Cell migration and invasion were evaluated by scratch assay and Transwell assay.Cell apoptosis was evaluated by Hoechst33342 staining and Annexin-V/PI double staining assay.Bax,Bcl-2 and Caspase-3 mRNA expression were detected using RT-qPCR.The protein expressions of JAK1,p-JAK1,STAT3,p-STAT3 and MMP9 were detected by Western blot.Results Compared with the control group,both Tongguanteng injection and extract significantly decreased the survival rate of MNNG/HOS and Saos-2 cells,inhibited cell clone formation,migration,and invasion,induced cell apoptosis(P<0.05,P<0.01),promoted Bax mRNA and protein expression,inhibited Bcl-2 mRNA and protein expression,and up-regulated Caspase-3 mRNA and Cleaved Caspase-3 protein expression.Tongguanteng injection could significantly down-regulate the expressions of p-JAK1,p-STAT3 and MMP9 protein expression in Saos-2 cells(P<0.05,P<0.01).Conclusion Both Tongguanteng injection and Tongguanteng extract can significantly inhibit proliferation,migration and invasion of human osteosarcoma MNNG/HOS and Saos-2 cells,and induce apoptosis,with no significant difference in anti-tumor effect.The mechanism may be related to the inhibition of the activation of JAK1/STAT3 signaling pathway.
