Expression of Vasculoendothelial Growth Factor in Distraction Osteogenesis of Rat Tibia.
- Author:
Tae Joon CHO
1
;
In Ho CHOI
;
Chin Youb CHUNG
;
Won Joon YOO
;
Hyeyoun SUNG
Author Information
1. Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- MeSH:
Animals;
Bone and Bones;
Immunohistochemistry;
Osteoblasts;
Osteogenesis, Distraction*;
Polymerase Chain Reaction;
Rats*;
Rats, Sprague-Dawley;
Reverse Transcription;
RNA;
RNA, Messenger;
Tibia*;
Vascular Endothelial Growth Factor A
- From:Journal of Korean Orthopaedic Research Society
2001;4(2):114-120
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: In order to delineate its function in distraction osteogenesis, the temporal and spatial expression patterns of vasculoendothelial growth factor(VEGF) mRNA and protein were investigated. MATERIALS AND METHODS: Distraction osteogenesis was carried out in the tibiae of Sprague-Dawley rat using bilateral monofixators. Total RNA was prepared from the bone tissue including distraction gap at postoperative 1, 2, 3, 4 weeks and in unoperative one. The temporal pattern of mRNA expression was observed using reverse transcription - polymerase chain reaction. Spatial expression of mRNA and protein of VEGF were observed using in-situ hybridization and immunohistochemistry. RESULTS: VEGF mRNA was expressed throughout the whole procedure, and the relative dominance of splicing variants was changing during distraction osteogenesis. VEGF expression of both mRNA and protein was highest in the immature osteoblasts at the primary matrix front. CONCLUSION: These data suggest that VEGF plays an important role in distraction osteogenesis, not only of angiogenesis but also of osteoblastic differentiation.
