Effects of TGF- beta 3 pretreatment in vitro on the differentiation of rabbit mesenchymal stem cell in vivo.
- Author:
Hyeong Geun PARK
1
;
So Ra PARK
;
Han Jo LIM
;
Byoung Hyun MIN
Author Information
1. Department of Molecular Science and Technology, Ajou University, Suwon, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Chondrogenic differentiation;
Rabbit mesenchymal stem cell;
Osteogenic differentiation;
PGA;
Tissue engineering;
TGF- beta 3
- MeSH:
Animals;
Cartilage;
Immunohistochemistry;
Mesenchymal Stromal Cells*;
Mice;
Mice, Nude;
Tissue Engineering
- From:Journal of Korean Orthopaedic Research Society
2001;4(2):167-176
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Bone and cartilage were manufactured by using tissue engineering of mesenchymal stem cell (MSC) which can differentiate into variety of cell types. MATERIAL AND METHOD: MSC was isolated and cultured from the rabbit weighing 500g, and it was seeded into PGA mesh and pre-cultured for 1 week with different TGF- beta 3 treated conditions. It was implanted into nude mice and tissues generated were recovered from 1, 2, 3, 4, 8 ,and 12 weeks respectively. Degree of bone and cartilage formation was analyzed with histology and immunohistochemistry assay. RESULT: Pre-culture condition with TGF- beta 3 treatment showed early start of chondrogenic differentiation, and degree of bone and cartilage formation was promoted as time passed. But both of the cases differentiated into complete bone after 12 weeks. CONCLUSION: The results show that pretreatment of TGF- beta 3 promotes the differentiation process in vivo condition under the in vivo system where MSC differentiate into bone via cartilage formation.
