Experimental Study of Inhibitory Effect of Kangxian Recipe on TGF-β1 Induced Hepatocyte Apoptosis
- Author:
Li-Huang ZHANG
1
;
Jia-Geng WU
;
Hang-Ping YAO
;
Min-Wei LI
Author Information
1. 浙江大学附属第一医院
- From:
Chinese journal of integrative medicine
2001;7(1):29-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the inhibitory effect of Kangxian Recipe (KXR) on TGF-β1 induced hepatocyte apoptosis. Methods: The in vitro model of hepatocyte apoptosis was established by cell biologic methods, utilizing the characteristics of TGF-β1 to observe the inhibitory effect of KXR on hepatocyte apoptosis. Results: TGF-β1 induced apoptosis of hepatocyte in a dose-dependent manner. The apoptosis rate of 2.2.15 cells was 63% when 500 ng/L TGF-β1 was used, while for HepG2 cell, it was merely 44%. After treatment of 20 ng/L KXR, the apoptosis rate of the two kinds of cell lines lowered to 33% and 24% respectively. The inhibiton rate of both groups was about 50%. Conclusion: KXR had strong inhibitory effect on hepatocyte apoptosis induced by TGF-β1.
