Expression and clinical significance of inhibitor of differentiation family in chronic my-eloid leukemia
10.12354/j.issn.1000-8179.2024.20240762
- VernacularTitle:分化抑制因子家族在慢性髓系白血病中的表达及临床意义
- Author:
Zhou JINGDONG
1
;
Xie FEI
;
Yuan QIAN
;
Guo HONG
;
Lin JIANG
;
Zhang TINGJUAN
;
Qian JUN
Author Information
1. 江苏大学附属人民医院血液科,镇江市血液病临床医学研究中心,镇江市血液系统恶性肿瘤精准诊断与治疗重点实验室(江苏省镇江市 212002)
- Keywords:
chronic myeloid leukemia(CML);
inhibitor of differentiation(ID);
expression;
methylation;
clinical significance
- From:
Chinese Journal of Clinical Oncology
2024;51(14):710-715
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression patterns of inhibitor of differentiation(ID)family in patients with chronic myeloid leukemia(CML)and analyze their clinical implications.Methods:Quantitative PCR and quantitative methylation-specific PCR were conducted to de-tect the transcript levels of ID2/ID3/ID4 and the methylation levels of ID4 in the bone marrow mononuclear cells of non-hematological ma-lignancies(acting as controls)and patients with CML treated at The Affiliated People's Hospital of Jiangsu University from January 2010 to December 2017.The clinical implications of ID family alterations were further analyzed.Results:ID2 and ID3 expression was significantly up regulated(P<0.001 and P<0.05,respectively),whereas ID4 expression was markedly down regulated in patients with CML(P<0.01).The re-ceiver operating characteristic curve demonstrated that the ID2 transcript level is a potential biomarker for distinguishing CML from controls(AUC=0.895,P<0.001).The frequency of ID4 promoter methylation in patients with CML was drastically higher than that in the controls(P=0.001).Moreover,ID4 methylation was negatively correlated with ID4 expression in patients with CML(r=-0.424,P=0.002).Clinically,CML with high ID2 expression occurred more frequently in males(P=0.040).Patients with low ID4 expression or high ID4 methylation showed a markedly higher frequency of an accelerated phase/blast crisis(P=0.003 and P<0.001,respectively).In addition,patients with CML in an accelerated phase/blast crisis exhibited markedly lower ID4 expression and higher ID4 methylation levels than those in the chronic phase(both P<0.001).Furthermore,univariate and multiple Logistic regression analyses revealed that the ID4 methylation level was an inde-pendent risk factor for CML progression(P=0.007).Conclusions:The ID family was differentially expressed in patients with CML;specifically,ID2 and ID3 expression was significantly increased,whereas ID4 expression was markedly decreased and correlated with ID4 promoter hy-permethylation.Hence,ID4 expression and methylation are confirmed to be associated with CML progression,and ID4 methylation could be an independent risk factor for CML progression.
