Study on the effect of inflammatory factors and CD4+and CD8+T cells in vaginal lavage in high-grade cervical squamous intraepithelial lesions and early cervical cancer
10.12354/j.issn.1000-8179.2024.20240349
- VernacularTitle:阴道灌洗液中炎症因子与CD4+和CD8+T细胞在高级别宫颈鳞状上皮内病变与早期宫颈癌发病中的作用研究
- Author:
Wang LAN
1
,
2
;
Tang JUAN
;
Yu GUANGYU
;
Ding GUOYAN
;
Yao TINGTING
Author Information
1. 桂林市人民医院妇科(广西壮族自治区 桂林市 541002)
2. 重庆医科大学研究生院
- Keywords:
high-grade cervical squamous intraepithelial lesions(HSIL);
cervical cancer(CC);
vaginal lavage;
CD4+and CD8+T cells;
local inflammatory factors
- From:
Chinese Journal of Clinical Oncology
2024;51(7):337-341
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of inflammatory factors(IL-2,INF-γ,IL-10,TNF-α)and CD4+and CD8+T cells in vaginal lavage in high-grade cervical squamous intraepithelial lesions(HSIL)and early cervical cancer(CC).Methods:To collect clinical data of HSIL(n=120)and early CC patients(n=44)after high-risk human papilloma virus(HR-HPV)infection.And healthy middle-aged women not infected with HR-HPV were randomly selected as control.The clinical data of the subjects in three groups were compared,inflammatory factors distribution and ratio of CD4+and CD8+T cells in serum and vaginal lavage fluid were compared.Multivariate Logistic regression performed to analyze the influencing factors of HSIL progression to early CC,ROC and calibration plot were drawn to evaluate the model.Results:The difference was not statistically significant in serum IL-2,INF-γ,IL-10,TNF-α levels,CD4+T cell distribution,CD8+T cell distribution and CD4+/CD8+ratio in sub-jects of control group,HSIL group and early CC group(P>0.05).The difference was statistically significant in the vaginal lavage levels of IL-2,INF-γ,IL-10,TNF-α levels,CD4+T cell distribution,CD8+T cell distribution and CD4+/CD8+ratio(P<0.05),plus the level of HSIL patients was higher than that of control subjects(P<0.05).Logistic regression analysis showed that parity>2(OR=3.119,95%CI:4.353～6.737)and the percentage of CD4+T cells in vaginal lavage fluid(OR=0.327,95%CI:0.188-0.478)in model 2(P<0.001,AUC=0.908),CD4+/CD8+(OR=0.809,95%CI:0.356-1.868)(P<0.001,AUC=0.873)in model 3 has an independent influence on the development of HSIL to early CC;the difference is not significant(Z=1.550 4,P=0.121)in ROC curves of the two models.CD4+/CD8+ratio as only one indictor in Model 3 can be good predic-tion,and the calibration curve of this model is close to the standard curve.Conclusions:After HR-HPV infection,the systemic immune status does not participate in HSIL and HSIL progression to early CC,but the cervical local immune status is involved,in which CD4+/CD8+T cell ratio is an independent protective factor.
