Molecular diagnosis of Helicobacter pylori antimicrobial resistance in the population of Wuwei City,China,an area with a high incidence of gastric cancer
10.12354/j.issn.1000-8179.2024.20240376
- VernacularTitle:胃癌高发区武威市人群幽门螺杆菌抗生素耐药性分子诊断研究
- Author:
Lu LINZHI
1
;
Li SHIHUA
;
Zhao GUANGYUAN
;
Liu JINDIAN
;
Ning XIUMEI
;
Wang XINGHUA
;
Wang XINGCHANG
;
Qin TIANYAN
Author Information
1. 甘肃省武威肿瘤医院消化科(甘肃省武威市 733000)
- Keywords:
Helicobacter pylori(HP);
CYP2C19 gene;
gastric cancer;
drug resistance
- From:
Chinese Journal of Clinical Oncology
2024;51(7):331-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the molecular characteristics of antibiotic resistance in Helicobacter pylori(HP)and provide a molecular bio-logical basis for clinical eradication of HP by means of rational antibiotic use.Methods:From February 2019 to November 2023,1,144 pa-tients at the Gansu Wuwei Cancer Hospital who tested positive for HP using the 14C-urea breath test were enrolled in the study.Antibiotic resistance and related molecular characteristics of HP,and CYP2C19 polymorphisms in the patients were detected by diffusion drug suscept-ibility testing,drug resistance gene testing,and next-generation sequencing,respectively.Results:Among the six antibiotics assessed,the resistance rate and the prevalence of resistance genes(rdxA)were highest for metronidazole(92.00%and 86.12%,respectively),and lowest for amoxicillin(Pbp1)(11.78%and 37.11%,respectively).The prevalence of CYP2C19 alleles showed that 46.77%,44.58%,and 8.65%of par-ticipants were fast,medium,and slow metabolizers,respectively.Of the participants,148(18.55%)had immunohistochemical sphericity.The eradication rate of HP lower using conventional treatment regimens than that using personalized treatment regimens(χ2=8.627,P=0.003).The HP eradication rate was higher among patients with a first diagnosis of drug resistancebased on molecular testing,than that in patients undergoing retreatment(χ2=6.242,P=0.012).Conclusions:The prevalence of amoxicillin-resistant HP is low in Wuwei City,which has a high incidence of gastric cancer.Molecular diagnosis of antimicrobial resistance could improve the HP eradication rate and provide a refer-ence for rational use of antibiotics in clinical practice.
