Cytoplasmic linker protein 170 inhibits papillary thyroid cancer cell metastasis through the TGF-β pathway
10.12354/j.issn.1000-8179.2024.20240154
- VernacularTitle:CLIP170通过TGF-β通路抑制甲状腺乳头状癌的转移
- Author:
Ma BINYUAN
1
;
Xu YAXIN
;
Pan YUNYAN
;
Wu YANI
;
Gao HONGWEI
Author Information
1. 兰州大学第二医院检验医学中心(兰州市 731000)
- Keywords:
cytoplasmic linker protein 170(CLIP170);
papillary thyroid cancer(PTC);
metastasis;
epithelial-mesenchymal transition(EMT);
TGF-β pathway
- From:
Chinese Journal of Clinical Oncology
2024;51(5):217-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether cytoplasmic linker protein 170(CLIP170)affects papillary thyroid cancer(PTC)cell metastasis and invasion and clarify the underlying mechanisms.Methods:We analyzed CLIP170 expression levels in PTC using GEO and TCGA data and con-structed CLIP170 knockdown(CLIP170KD)cells using lentiviral transfection.Then,we evaluated their functions through Transwell transfer and invasion assays.We assessed how CLIP170 affected the cellular actin structure via immunofluorescence analysis.We detected transforming growth factor-β 1(TGF-β1)release in the cell culture medium using enzyme-linked immunosorbent assay(ELISA).We also assessed epithelial-mesenchymal transition(EMT)and TGF-β signaling pathway molecule expression using immunoblotting and reverse-transcription quantitat-ive fluorescence PCR and validated the results in a nude mouse lung metastasis model.Results:CLIP170 expression level in PTC was lower than that in normal thyroid tissue.Regarding the function,CLIP170KD significantly enhanced PTC cell metastasis both in vitro and in vivo.Re-garding the underlying mechanism,CLIP170KD triggered TGF-β pathway activation,subsequently promoted tumor cell migration and invasion.The inhibitor of TGF-β effectively inhibited TGF-β activation,and this inhibition significantly reversed the CLIP170KD-induced tumor metastasis.Conclusions:CLIP170 could be a promising therapeutic target to mitigate metastatic tendencies in PTC.
