Gene-expression profiling of titanium-cell interaction.
- Author:
Chang Su KIM
1
;
Jung Won HWANG
;
Jae Jun RYU
;
Sang Wan SHIN
;
Sung Hwa SOHN
;
Ki Nam KIM
;
Meyoung Kon KIM
Author Information
1. Department of Prosthodontics, Seoul National University Bundang Hospital, Korea.
- Publication Type:Original Article
- Keywords:
Titanium surfaces;
expression profiling;
cDNA microarray;
MG63
- MeSH:
Bone Morphogenetic Proteins;
Cell Line;
Collagen;
Dental Materials;
DNA, Complementary;
Genes, Regulator;
Humans;
Oligonucleotide Array Sequence Analysis;
Osseointegration;
Osteoblasts;
Osteogenesis;
Receptor, Fibroblast Growth Factor, Type 4;
RNA;
Somatomedins;
Titanium
- From:The Journal of Korean Academy of Prosthodontics
2005;43(3):393-408
- CountryRepublic of Korea
- Language:English
-
Abstract:
STATEMENT OF PROBLEM: In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function. PURPOSE: This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63). MATERIALS AND METHODS: MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit and acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements). RESULTS: The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces. CONCLUSION: The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.
