Preparation of Biomimetic Dual-Mode Tumor-Targeted Nanobubbles as Ultrasound Contrast Agents and Its Function
10.3969/j.issn.1005-5185.2024.06.002
- VernacularTitle:仿生双模态肿瘤靶向纳米气泡超声造影剂的制备及其功能
- Author:
Yu WANG
1
;
Chao LI
;
Weijing WANG
;
Yunyou DUAN
;
Hengli YANG
Author Information
1. 西安医学院第二附属医院超声科,陕西 西安 710038
- Keywords:
Biomimetic;
Immune escape;
Tumor-targeted nanobubbles;
Bimodal imaging
- From:
Chinese Journal of Medical Imaging
2024;32(6):533-538
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To prepare biomimetic targeted nanobubbles(NBs)named IR780-RBCM@NBs and explore the ability of performing ultrasound enhancement,escaping immune surveillance and targeting tumor cells.Materials and Methods The red blood cell membrane(RBCM)was extracted from mouse blood,the IR780-RBCM@NBs were prepared by modified membrane hydration method,the physical and chemical properties of nanobubbles were detected.In vitro,self-made equipment was used to test the contrast-enhanced ultrasound imaging ability of the nanobubbles.Laser confocal fluorescence microscopy was used to observe the characteristics of the nanobubbles retaining CD47 on the RBCM surface,and further to observe the phagocytosis of the nanobubbles by macrophages and the targeting ability of the nanobubbles to different tumor cells.Results The newly prepared IR780-RBCM@NBs had the appearance of emulsion,the particle size was about(522.4±58.6)nm and the dispersity was good.It was spherical in uniform size under the microscope.IR-780 could be detected on the nanobubbles,which had the dual-mode imaging capabilities of near-infrared fluorescence imaging and contrast-enhanced ultrasound imaging.The RBCM-specific protein CD47 could be detected on the surface of the nanobubbles.The nanobubbles exhibited excellent immune phagocytic escape function and high efficiency to target different tumor cells.Conclusion Newly prepared biomimetic targeted IR780-RBCM@NBs,with good performance,can escape immune phagocytosis and efficiently target tumors,providing a new idea for molecular targeted accurate diagnosis and treatment of tumors.
