Effect and Mechanism of Uric Acid in Regulating Larval Growth and Development of Drosophila Melanogaster
10.3881/j.issn.1000-503X.16013
- VernacularTitle:尿酸对果蝇幼虫生长发育的影响及机制分析
- Author:
Ruidi ZHANG
1
;
Hongbin QIU
;
Jingtao WANG
;
Baosheng GUAN
;
Xue BAI
;
Xianglin YIN
Author Information
1. 佳木斯大学公共卫生学院,黑龙江佳木斯 154007
- Keywords:
uric acid;
Drosophila melanogaster;
reactive oxygen species;
forkhead box transcription factor;
mammalian target of rapamycin complex 1
- From:
Acta Academiae Medicinae Sinicae
2024;46(5):653-658
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and mechanism of uric acid(UA)in regulating the larval growth and development of Drosophila melanogaster.Methods A total of 1350 newly hatched first-instar larvae of wild-type Drosophila melanogaster(W11 18)were collected,and the Drosophila melanogaster model of hyperurice-mia was constructed with a high purine diet.The larvae were assigned into three groups(n=150):control(stand-ard corn meal medium),low-dose adenine(corn meal medium containing 0.05％adenine),and high-dose ad-enine(corn meal medium containing 0.10％adenine),and two parallel groups were set up.The growth and de-velopment of larvae in each group was observed,and the UA and hormone levels were measured.In addition,the expression levels of genes involved in growth and development were determined.Results Compared with the con-trol group,the low-and high-dose adenine groups showed elevated UA levels(both P＜0.001)and prolonged de-velopmental period(P=0.024,P＜0.001).The high-dose adenine group showed decreased survival rate,pupa-tion rate,and eclosion rate and elevated levels of juvenile hormone(JH)and 20-hydroxyecdysone(20E)(all P＜0.001).The PCR results showed that compared with the control group,high-dose adenine upregulated the mRNA levels of reactive oxygen species(ROS),forkhead box O(FOXO),and mammalian target of rapamycin(mTOR)while downregulating the mRNA levels of Sestrin,mTOR complex 1(mTORC1),and AMP-activated protein kinase(all P＜0.001).Conclusion High concentrations of UA may promote the expression of ROS/FOXO/mTORC1/mTOR signaling pathway by regulating the levels of JH and 20E,thereby inhibiting the larval growth and development of Drosophila melanogaster.
