Expression Levels and Regulation of Selenoprotein Genes in Patients With Coronavirus Disease 2019
10.3881/j.issn.1000-503X.15834
- VernacularTitle:硒蛋白基因在新型冠状病毒感染患者中的表达水平及调控作用
- Author:
Jing LI
1
;
Rongqiang ZHANG
;
Lingzhi ZHANG
;
Yan QI
;
Jie HAO
;
Aoyue HE
;
Xu ZHAO
;
Xiuqin LI
Author Information
1. 陕西中医药大学公共卫生学院流行病与卫生统计学教研室,陕西咸阳 712046
- Keywords:
coronavirus disease 2019;
selenoprotein;
expression and regulation;
Logistic regression
- From:
Acta Academiae Medicinae Sinicae
2024;46(3):316-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels of selenoprotein genes in the patients with coronavirus disease 2019(COVID-19)and the possible regulatory mechanisms.Methods The dataset GSE177477 was obtained from the Gene Expression Omnibus,consisting of a symptomatic group(n=11),an asymptomatic group(n=18),and a healthy control group(n=18).The dataset was preprocessed to screen the differentially expressed genes(DEG)related to COVID-19,and gene ontology functional annotation and Kyoto encyclopedia of genes and genomes enrichment analysis were performed for the DEGs.The protein-protein interaction network of DEGs was established,and multivariate Logistic regression was employed to analyze the effects of selenoprotein genes on the presence/absence of symptoms in the patients with COVID-19.Results Compared with the healthy control,the symptomatic COVID-19 patients presented up-regulated expression of GPX1,GPX4,GPX6,DIO2,TXNRD1,SELENOF,SELENOK,SELENOS,SELENOT,and SELENOW and down-regulated ex-pression of TXNRD2 and SELENON(all P＜0.05).The asymptomatic patients showcased up-regulated expres-sion of GPX2,SELENOI,SELENOO,SELENOS,SELENOT,and SELENOW and down-regulated expression of SELP(all P＜0.05).The results of multivariate Logistic regression analysis showed that the abnormally high expression of GPX1(OR=0.067,95％CI=0.005-0.904,P=0.042)and SELENON(OR=56.663,95％CI=3.114-856.999,P=0.006)was the risk factor for symptomatic COVID-19,and the abnormally high expres-sion of SELP was a risk factor for asymptomatic COVID-19(OR=15.000,95％CI=2.537-88.701,P=0.003).Conclusions Selenoprotein genes with differential expression are involved in the regulation of COVID-19 development.The findings provide a new reference for the prevention and treatment of COVID-19.
