Effects of Platelet-Rich Plasma-Derived Exosomes on Proliferation and Migration of Tendon Stem/Progenitor Cell
10.3881/j.issn.1000-503X.15890
- VernacularTitle:富血小板血浆源性外泌体对肌腱干/祖细胞增殖及迁移能力的影响
- Author:
Molin LI
1
,
2
;
Yaqiong ZHU
;
Yufei DING
;
Dan YI
;
Naiqiao GE
;
Siming CHEN
;
Yuexiang WANG
Author Information
1. 解放军医学院,北京 100853
2. 中国人民解放军总医院第一医学中心超声诊断科,北京 100853
- Keywords:
platelet-rich plasma;
exosome;
tendon stem/progenitor cell;
proliferation;
migration;
rotator cuff injury
- From:
Acta Academiae Medicinae Sinicae
2024;46(3):307-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of platelet-rich plasma-derived exosomes(PRP-Exos)on the proliferation and migration of tendon stem/progenitor cell(TSPC).Methods PRP-Exos were extracted through the combination of polymer-based precipitation and ultracentrifugation.The morphology,concentration,and particle size of PRP-Exos were identified by transmission electron microscopy and nanoparticle tracking analysis.The expression levels of surface marker proteins on PRP-Exos and platelet membrane glycoproteins were deter-mined by Western blot analysis.Rat TSPC was extracted and cultured,and the expression of surface marker mol-ecules on TSPC was detected using flow cytometry and immunofluorescence staining.The proliferation of TSPC in-fluenced by PRP-Exos was evaluated using CCK-8 assay and EdU assay.The effect of PRP-Exos on the migration of TSPC was evaluated by cell scratch assay and Transwell assay.Results The extracted PRP-Exos exhibit typi-cal saucer-like structures,with a concentration of 4.9 ×1011 particles/mL,an average particle size of(132.2±56.8)nm,and surface expression of CD9,CD63 and CD41.The extracted TSPC expressed the CD44 pro-tein.PRP-Exos can be taken up by TSPC,and after co-cultured for 48 h,concentrations of 50 and 100 μg/mL of PRP-Exos significantly promoted the proliferation of TSPC(both P＜0.001),with no statistical difference be-tween the two concentrations(P=0.283).Additionally,after co-cultured for 24 h,50 μg/mL of PRP-Exos significantly promoted the migration of TSPC(P＜0.001).Conclusion Under in vitro culture conditions,PRP-Exos significantly promote the proliferation and migration of rat TSPC.
