A Study on Cellular Matrix Via Cell Extraction from Bovine Pericardium
- VernacularTitle:牛心包脱细胞基质的研制
- Author:
Jian-Ye ZHOU
1
;
Sheng-Shou HU
;
Bing REN
;
Guo-Qi QI
Author Information
1. Fuwai Cardiovascular Institute, CAMS and PUMC
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):193-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain an acellular matrix from bovine pericardial tissue, as a scaffold for tissue engineering of heart valves or cardiovascular patching applications. Methods A four step detergent and enzyme link extraction procedure was practiced in this study. The mechanic properties of the acellular matrix were evaluated, and the components were analyzed biochemically. Results HE staining confirmed the removal of cells and Von Gieson staining showed the integrality of collagen and elastin. Biochemical analysis demonstrated the retention of collagen and some glycosaminoglycans while the percentage of the soluble proteins reduced slightly. The tissue fracture strength and shrinkage temperature made no significant differ ence after extraction. Conclusions The extraction process is effective to remove cells from bovine pericardial tissue while maintains its mechanical strength. This approach may eventually lead to a scaffold for heart valves and cardiovascular patching applications in tissue engineering.
