Killing Effect of Human Pulmonary Adenocarcinoma Cells with
- VernacularTitle:自杀基因CD、TK的共表达对人肺腺癌细胞的杀伤作用
- Author:
Hui-Qing CAO
1
;
Yong ZHAO
;
Xian-Min MENG
;
Jin-Feng DING
Author Information
1. FuWai Heart Hospital
- From:
Acta Academiae Medicinae Sinicae
2001;23(2):168-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the different killing effect to human pulmonary adenocarcinoma cell line cells GLC-82 with coexpressed double suicide genes compared with single gene. Methods Recombinant expression vectors containing CD (cytosine deaminase) and/or TK(thymidine kinase) gene under CMV promoter were constructed successfully. The vectors were transfected to GLC-82 tumor cell lines by use of lipofectamine. The clones were picked out after G418 selection. Extraneous gene integration and expression were confirmed by PCR and semi-quantitative RT-PCR. The cytotoxicity to these transgenic cells under treatment with 5-Fc and GCV were measured by MT1 assays. Results Double and single suicide gene transfer were both stably expressed in GLC-82 cells . The cytotoxic effects of co-expressed TK-CD genes were superior than that of the single gene. Conclusion The CD + TK/5-Fc + GCV coexpression system is more effective for killing effect of tumor cells than CD/5-Fc or TK/GCV system alone.
